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MABN691 Anti-GPI Antibody, clone 1B7D7

Anti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence.

Anti-GPI Antibody, clone 1B7D7: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: AMF, NLK, PGI, PHI, GNPI, SA-36, GPI UniProt Number: P06744
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MABN691
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IHC, IFMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN691
      DescriptionAnti-GPI Antibody, clone 1B7D7
      Alternate Names
      • Glucose-6-phosphate isomerase
      • Autocrine motility factor
      • Neuroleukin
      • Phosphoglucose isomerase
      • Phosphohexose isomerase
      • Sperm antigen 36
      Background InformationGlucose-6-phosphate isomerase (GPI) alternatively known as Autocrine motility factor (AMF), Neuroleukin (NLK), Phosphoglucose isomerase (PGI), Phosphohexose isomerase (PHI) or Sperm antigen 36 (SA-36) and encoded by the gene name GPI is major enzyme in glycolysis and gluconeogenesis. Glucose-6-phosphate isomerase rearranges glucose-6-phosphate (from the phosphorylation of glucose via hexokinases) into fructose-6-phosphate (F6P) as the second step in glycolysis. Interestingly however, Glucose-6-phosphate isomerase plays an entirely different sort of role outside of the cell than when it is inside being part of glycolysis. When secreted, Glucose-6-phosphate isomerase acts as an autocrine motility factor for various cancers and is critical for metastasis, while when secreted in nervous tissue it acts as a neurotrophic factor for sensory and spinal neurons and in lymphocytes it acts as a lymphokine upon T cell ligand binding and stimulates B cells to secrete immunoglobulin. Mutations in Glucose-6-phosphate isomerase are the second most frequent cause of inherited glycolytic-enzymopathy in humans. This autosomal recessive disorder is characterized by anon-spherocytic anemia of variable severity which can present with neuromuscular dysfunctions defined by muscle weakness and mental retardation. EMD-Millipore’s Anti-Glucose-6-phosphate isomerase monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HepG2, SMMC-7721 and rat liver tissue and in paraffin embedded immunohistochemistry on human kidney and fluorescent immunocytochemistry on L-O2 cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • HepG2, SMMC-7721, and rat liver lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GPI Antibody, clone 1B7D7 is a highly specific mouse monoclonal antibody, that targets Glucose-6-phosphate isomerase & has been tested in western blotting, IHC & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in human kidney tissue.

      Immunofluorescent Analysis: A 1:200-1,000 dilution from a representative lot detected GPI in L-02 cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human GPI expressed in E. Coli.
      Clone1B7D7
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • AMF
      • NLK
      • PGI
      • PHI
      • GNPI
      • SA-36
      • GPI
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~56 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2, SMMC-7721, and rat liver lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GPI in HepG2, SMMC-7721, and rat liver lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABN691 04053252917431

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies