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MABE1052 Anti-GMPS Antibody, clone 1G6.1

Anti-GMPS, clone 1G6.1, Cat. No. MABE1052, is a highly specific mouse monoclonal antibody that targets GMPS and has been tested in Western Blotting.

Anti-GMPS Antibody, clone 1G6.1: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: GMPS UniProt Number: P49915
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MABE1052
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1052
      DescriptionAnti-GMPS Antibody, clone 1G6.1
      Alternate Names
      • GMP synthase [glutamine-hydrolyzing]
      • Glutamine amidotransferase
      • GMP synthetase
      Background InformationGMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2; UniProt P49915; also known as Glutamine amidotransferase, GMP synthetase) is encoded by the GMPS gene (Gene ID 8833) in human. De novo biosynthesis of Guanosine 5'-monophosphate (GMP) is accomplished by reactions catalyzed by several enzymes. Inositol monophosphate (IMP) dehydrogenase 1 and 2 (IMPDH1 and IMPDH2) convert IMP into xanthosine monophosphate (XMP), GMP synthetase (GMPS) then converts XMP into GMP, while guanosine monophosphate reductase (GMPR) catalyzes the reversible conversion of GMP to IMP to fuel back into both the AMP and GMP synthesis pathways. GMPS is a bifunctional two-domain N-type pyrophosphatase whose N-terminal glutaminase domain (a.a. 27-216) generates ammonia from glutamine, while its C-terminal synthetase domain (a.a. 27-435) adenylylates XMP on the xanthine C2 oxygen, thereby priming XMP for attack by a nitrogen nucleophile to form GMP. Human GMPS activity is subjected to an allosteric regulation and displays positive cooperativity for the substrate XMP. GMPS is normally sequestered in the cytoplasm, but translocates to the nucleus in response to genotoxic stress or nucleotide deprivation. GMPS nuclear translocation plays an important role in USP7-mediated stabilization of p53, where p53 is transferred from MDM2 to the GMPS-USP7 deubiquitylation complex.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GMPS, clone 1G6.1, Cat. No. MABE1052, is a highly specific mouse monoclonal antibody that targets GMPS and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant human GMPS ATP-PPase domain fragment.
      Clone1G6.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1G6.1 targets an epitope within the GMPS ATP-PPase domain region present in both spliced isoforms of human GMPS reported by UniProt (P49915).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GMPS
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~70 kDa observed. 76.58/65.80 kDa (isoform 1/2) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected GMPS in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE1052 04054839090240