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07-172-I Anti-FHIT

Anti-FHIT, Cat. No. 07-172-I, is a highly specific rabbit polyclonal antibody that targets FHIT and has been tested in Immunoprecipitation and Western Blotting.

Anti-FHIT: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: FHIT UniProt Number: P49789
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07-172-I
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-172-I
      Replaces07-172
      DescriptionAnti-FHIT
      Alternate Names
      • Bis(5'-adenosyl)-triphosphatase
      • AP3A hydrolase
      • AP3Aase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase
      • Dinucleosidetriphosphatase
      • Fragile histidine triad protein
      Background InformationBis(5'-adenosyl)-triphosphatase (EC 3.6.1.29; UniProt P49789; also known as AP3A hydrolase, AP3Aase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein) is encoded by the FHIT gene (Gene ID 2722) in human. Common fragile sites (CFSs) are large genomic loci that display chromosomal instability in response to genotoxic or replicative stress. Many CFSs harbor genes that contribute to cancer development and are non-randomly altered in cancers through genomic alteration. The Fragile histidine triad (FHIT) gene at FRA3B is one of the earliest and most frequently altered genes in the majority of human cancers. FHIT protein plays a role in apoptosis and prevention of the epithelial-mesenchymal transition (EMT). A FHIT-upregulated microRNA, miR-30c, has been identified to mediate the suppression of EMT and metastasis by directly targeting metastasis genes Metadherin (MTDH), High-mobility group AT-hook 2 (HMGA2), and the mesenchymal markers, Vimentin (VIM) and Fibronectin (FN1), in human lung cancer. In addition, FHIT protein also plays an important role in maintaining genome stability, loss of FHIT protein function leads to nucleotide imbalance, spontaneous replication stress, and DNA breaks. Because FHIT loss-induced DNA damage is checkpoint-independent, cells accumulate further DNA damage during subsequent cell cycles, accruing global genome instability that could facilitate oncogenic mutation acquisition and expedite clonal expansion.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-FHIT, Cat. No. 07-172-I, is a highly specific rabbit polyclonal antibody that targets FHIT and has been tested in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 5 µg from a representative lot immunoprecipited FHIT from 500 µg of A549 cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a C-terminal region sequence of human FHIT.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody targets a C-terminal epitope region outside the HIT domain.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FHIT
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~17 kDa observed. 16.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549 cell lysate.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected FHIT in 10 µg of A549 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      07-172-I 04054839069116