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ABE1379 Anti-F-box/WD repeat-containing protein 7 Antibody

This Anti-F-box/WD repeat-containing protein 7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of F-box/WD repeat-containing protein 7.

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Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: FBXW7, FBW7, FBX30, SEL10 UniProt Number: Q969H0
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ABE1379
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1379
      DescriptionAnti-F-box/WD repeat-containing protein 7 Antibody
      Alternate Names
      • Archipelago homolog
      • F-box/WD repeat-containing protein 7
      • hAgo
      • F-box and WD-40 domain-containing protein 7
      • F-box protein FBX30
      • SEL-10
      • hCdc4
      Background InformationF-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NFκB2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-F-box/WD repeat-containing protein 7 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of F-box/WD repeat-containing protein 7.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected F-box/WD repeat-containing protein 7 in human cerebellum and human pancreas tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human F-box/WD repeat-containing protein 7.
      EpitopeInternal
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityAlthough immunogen sequence is present in all three spliced isoforms, both target band size by Western blotting and cytoplasmic staining pattern by immunohistochemistry are consistent with the detection of isoform 2 (Archipelago beta, FBW7beta, 69K) by this polyclonal antibody.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Bovine, Canine, Feline, Porcine, Rhesus Macaque, Gorilla, Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FBXW7
      • FBW7
      • FBX30
      • SEL10
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~70 kDa observed. 79.7 kDa (isoform 1; Archipelago alpha, FBW7alpha, 110K, common), 70.3 kDa (isoform 2; Archipelago beta, FBW7beta, 69K), and 66.1 kDa (isoform 3; Archipelago gamma, FBW7gamma, Hippocampal) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-10A human breast epithelial cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected F-box/WD repeat-containing protein 7 in 10 µg of human breast cancer MDA-MB-231 and non-tumorigenic MCF-10A breast epithelial cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE1379 04055977274813

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies