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PC283 Anti-ETO (Ab-1) Rabbit pAb

Anti-ETO (Ab-1) Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-MTG8

PC283
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes human ETO in HEL cells.
      Catalogue NumberPC283
      Brand Family Calbiochem®
      SynonymsAnti-MTG8
      References
      ReferencesWang, J., et al. 1997. Cancer Res. 57, 2951.
      Yergeau, D.A., et al. 1997. Nat. Genet. 15, 303.
      Klampfer, L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14059.
      Rhoades, K.L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11895.
      Era, T., et al. 1995. Genes Chromosomes Cancer 13, 25.
      Erickson, P.F., et al. 1994. Cancer Res. 54, 1782.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Positive controlHEL cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Frozen Sections
      Not Immunofluorescence
      Not Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
      Immunoprecipitation (1 µg/sample)
      Frozen Sections (not recommended)
      Paraffin Sections (not recommended)
      Immunofluorescence (not recommended)
      Application CommentsAntibody should be tritrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of the C-terminal 212 amino acids of human ETO
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      PC283 0

      Documentation

      Anti-ETO (Ab-1) Rabbit pAb SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-ETO (Ab-1) Rabbit pAb Analysenzertifikate

      TitelChargennummer
      PC283

      Literatur

      Übersicht
      Wang, J., et al. 1997. Cancer Res. 57, 2951.
      Yergeau, D.A., et al. 1997. Nat. Genet. 15, 303.
      Klampfer, L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14059.
      Rhoades, K.L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11895.
      Era, T., et al. 1995. Genes Chromosomes Cancer 13, 25.
      Erickson, P.F., et al. 1994. Cancer Res. 54, 1782.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      SynonymsAnti-MTG8
      ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
      Immunoprecipitation (1 µg/sample)
      Frozen Sections (not recommended)
      Paraffin Sections (not recommended)
      Immunofluorescence (not recommended)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ETO protein.
      BackgroundOne of the most frequent translocations seen in acute myeloid leukemias (AML) is that which occurs between the AML1 locus on chromosome 21 and a second locus on chromosome 8, the so called ETO/MTG8 (eight twenty one) locus. While the exact function of the normal ETO protein is unknown based on the high content of proline and threonine residues, the putative zinc fingers and the TAF110 homology domains, it is most likely that it functions as a transcription factor. The translocation between AML1 and ETO is observed in 40% of M2-subtype AML's . In the translocation the amino terminal end of AML1 becomes fused to an almost full length ETO protein. The resulting chimeric protein has multiple functions among which are the ability to transactivate the bcl-2 gene in U937 cells, and the M-CSF gene. Both of these activating functions are markedly different from the normal function of the fusion protein, which is the repression of transcription. The normal ETO protein may also has important functions on its own based on the observation of ETO transcripts in normal hematopoietic cells of different lineages and the presence of relatively high message levels in the developing brain as well as the observation that AML/ETO heterozygous mice die in midgestation from CNS hemorrhaging.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of the C-terminal 212 amino acids of human ETO
      IsotypeIgG
      Specieshuman, mouse
      Positive controlHEL cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be tritrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesWang, J., et al. 1997. Cancer Res. 57, 2951.
      Yergeau, D.A., et al. 1997. Nat. Genet. 15, 303.
      Klampfer, L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14059.
      Rhoades, K.L., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 11895.
      Era, T., et al. 1995. Genes Chromosomes Cancer 13, 25.
      Erickson, P.F., et al. 1994. Cancer Res. 54, 1782.