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48-602MAG
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The Early Restricted protein (Ea-R) of Epstein-Bar Virus is encoded by the viral BHFR1 gene. Ea-R is a 17 kDa protein (p17) with protein kinase activity. Ea-R-p17 is a functional homologue of the anti-apoptotic oncoprotein bcl-2, which is expressed during the switch from latency to lytic replication and may be involved in proliferation of EBV infected cells. However, Ea-R-p17 does not appear to be essential for virus replication or transformation of B cells in vitro.
References
Product Information
Format
Purified
HS Code
3002 15 90
Presentation
Purified immunoglobulin presented as a liquid in 0.02M PBS, 0.25M NaCl, with 0.1% sodium azide.
Anti-EBV EA-R-p17 Antibody, clone 5B11 is an antibody against EBV EA-R-p17 for use in ELISA, IF, IP & WB.
Key Applications
ELISA
Immunofluorescence
Immunoprecipitation
Western Blotting
Application Notes
Immunoblotting
Immunoprecipitation
Immunofluorescence
Optimal dilutions must be determined by the end user.
Biological Information
Clone
5B11
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Mouse
Specificity
Recognizes the 17 kDa Early Restricted Protein (Ea-R-p17) of Epstein-Barr Virus by immunoblot, immunoprecipitation and immunofluorescence.
Isotype
IgG2a
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Comparative analysis of the expression of the Epstein-Barr virus (EBV) anti-apoptotic gene BHRF1 in nasopharyngeal carcinoma and EBV-related lymphoid diseases Nicholls, J. et al. J Med Virol, 65:105-113 (2001)
2001
Herpesvirus papio encodes a functional homologue of the Epstein-Barr virus apoptosis suppressor, BHRF1. Meseda, C A, et al. J. Gen. Virol., 81: 1801-5 (2000)
1999
The human tumour virus Epstein-Barr virus (EBV) encodes a 17 kDa protein, BHRF1, which is a member of the BCL:-2 family and has been shown to suppress apoptosis. The role of this gene in the life-cycle of EBV has not been fully elucidated. In order to identify motifs conserved in herpesviruses and possibly shed light on its function we isolated a BHRF1 homologue from herpesvirus papio (cercopithecine herpesvirus-12) a closely related gammaherpesvirus of baboons. The gene, hvpBHRF1, also encodes a 17 kDa protein which shares 64% identity and 79% similarity with EBV BHRF1 at the amino acid level. In biological assays, hvpBHRF1 and BHRF1 conferred similar levels of protection on human keratinocytes induced to apoptose with cis-platin.
Functional differences between BHRF1, the Epstein-Barr virus-encoded Bcl-2 homologue, and Bcl-2 in human epithelial cells Dawson, C. et al. J Virol, 72:9016-9024 (1998)
1998
Epstein-Barr virus contributes to the malignant phenotype and to apoptosis resistance in Burkitt's lymphoma cell line Akata. J Komano, M Sugiura, K Takada Journal of virology
72
9150-6
1998
In the present study, we established an in vitro system representing the Burkitt's lymphoma (BL)-type Epstein-Barr virus (EBV) infection which is characterized by expression of EBV-determined nuclear antigen 1 (EBNA-1) and absence of EBNA-2 and latent membrane protein 1 (LMP1) expression. EBV-negative cell clones isolated from the EBV-positive BL line Akata were infected with an EBV recombinant carrying a selectable marker, and the following selection culture easily yielded EBV-infected clones. EBV-reinfected clones showed BL-type EBV expression and restored the capacity for growth on soft agar and tumorigenicity in SCID mice that were originally retained in parental EBV-positive Akata cells and lost in EBV-negative subclones. Moreover, it was found that EBV-positive cells were more resistant to apoptosis than were EBV-negative cells. EBV-infected cells expressed the bcl-2 protein, through which cells might become resistant to apoptosis, at a higher level than did uninfected cells. This is the first report that BL-type EBV infection confers apoptosis resistance even in the absence of expression of LMP1 and BHRF1, both of which are known to have an antiapoptotic function. Surprisingly, transfection of the EBNA-1 gene into EBV-negative Akata clones could not restore malignant phenotypes and apoptosis resistance, thus suggesting that EBNA-1 alone was not sufficient for conferring them. Our results suggest that the persistence of EBV in BL cells is required for the cells to be more malignant and apoptosis resistant, which underlines the oncogenic role of EBV in BL genesis.
Identification of an Epstein-Barr virus early gene encoding a second component of the restricted early antigen complex Pearson, G. et al. Virol, 160:151-161 (1987)
1987
