Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
AB10525
Sigma-AldrichAnti-EBF-3 Antibody
Anti-EBF-3, Cat. No. AB10525, is a rabbit polyclonal antibody that detects Early B-cell factor 3 (EBF3) and is tested for use in Dot Blot, Immunofluorescence, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
More>>Anti-EBF-3, Cat. No. AB10525, is a rabbit polyclonal antibody that detects Early B-cell factor 3 (EBF3) and is tested for use in Dot Blot, Immunofluorescence, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. Less<<
Anti-EBF-3 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Transcription factor COE3 (UniProt: O08791; also known as Early B-cell factor 3, EBF-3, Olf-1/EBF-like 2, O/E-2, OE-2, EBF-3) is encoded by the Ebf3 (also known as Coe3) gene (Gene ID: 13593) in murine species. EBFs are a family of highly conserved DNA-binding transcription factors with an atypical zinc-finger and helix-loop-helix motif. All members of the EBF family (EBF-2, EBF-3, and EBF-4) possess a highly conserved DNA-binding domain that is distinct from that of other known DNA-binding proteins. These proteins serve diverse functions in the development of multiple lineages, including neurons, B cells, and adipocytes. EBF-3 is highly expressed in adult olfactory epithelium. In the developing embryo, it is expressed in epithalamus, hypothalamus, brainstem, ventricular zones of mesencephalon, and in rhombencephalon. It is highly expressed in developing retina. EBF-3 recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'. EBF-3 is reported to act as a putative tumor suppressor gene in acute myeloid leukemia (AML) and other cancers. Silencing of the EBF3 locus has been observed in brain, colorectal, breast, liver, and bone tumor cell lines. Its reactivation has been reported following treatment with 5-aza-2 -deoxycytidine and Trichostatin A (TSA) and overexpression of EBF-3 is shown to result in cell cycle arrest and apoptosis. EBF3 promoter is reported to be hypermethylated in AML and several cancer cell lines, including HL-60, U937, and K562 cells. (Ref.: Tao, YF., et al. (2015). J. Exp. Clin. Cancer Res. 34(1); 4; Kim, J., et al. (2012). Int. J. Cancer. : 130(4); 817-826).
References
Product Information
Format
Affinity Purified
Control
Mouse E16 brain tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-EBF-3, Cat. No. AB10525, is a rabbit polyclonal antibody that detects Early B-cell factor 3 (EBF3) and is tested for use in Dot Blot, Immunofluorescence, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Key Applications
Western Blotting
Immunofluorescence
Immunohistochemistry (Paraffin)
Peptide Inhibition Assay
Dot Blot
Application Notes
Peptide Inhibition Assay: Target band detection in E16 Mouse brain tissue lysate was prevented by preblocking of a representative lot with the EBF-3 immunogen peptide.
Dot Blot Analysis: A 1:500 dilution from a representative lot detected EBF-3 peptide, but not EBF-1 or EBF-2 peptides.
Immunohistochemistry (Paraffin) Analysis: A representative lot detected EBF-3 in Immunohistochemistry applications.
Immunofluorescence Analysis: A representative lot detected EBF-3 in Immunofluorescence applications.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal half of Mouse Early B-cell factor 3 (EBF-3).
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This rabbit polyclonal antibody specifically detects Early B-cell factor 3 (EBF-3). It targets an epitope within 11 amino acids from the C-terminal half.
Species Reactivity
Mouse
Species Reactivity Note
Mouse. Predicted to react with Human, Monkey, Bovine, Rat based on 100% sequence homology.
~72 kDa observed; 64.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in E16 Mouse brain tissue lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected EBF-3 in E16 Mouse brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Activation of classical brown adipocytes in the adult human perirenal depot is highly correlated with PRDM16-EHMT1 complex expression. Nagano, G; Ohno, H; Oki, K; Kobuke, K; Shiwa, T; Yoneda, M; Kohno, N PloS one
10
e0122584
2015
Brown fat generates heat to protect against cold and obesity. Adrenergic stimulation activates the thermogenic program of brown adipocytes. Although the bioactivity of brown adipose tissue in adult humans had been assumed to very low, several studies using positron emission tomography-computed tomography (PET-CT) have detected bioactive brown adipose tissue in adult humans under cold exposure. In this study, we collected adipose tissues obtained from the perirenal regions of adult patients with pheochromocytoma (PHEO) or non-functioning adrenal tumors (NF). We demonstrated that perirenal brown adipocytes were activated in adult patients with PHEO. These cells had the molecular characteristics of classical brown fat rather than those of beige/brite fat. Expression of brown adipose tissue markers such as uncoupling protein 1 (UCP1) and cell death-inducing DFFA-like effector A (CIDEA) was highly correlated with the amounts of PRD1-BF-1-RIZ1 homologous domain-containing protein-16 (PRDM16) - euchromatic histone-lysine N-methyltransferase 1 (EHMT1) complex, the key transcriptional switch for brown fat development. These results provide novel insights into the reconstruction of human brown adipocytes and their therapeutic application against obesity and its complications such as type 2 diabetes.
