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ABT155
Sigma-AldrichAnti-Dynamin related protein 1 (Drp1) Antibody
This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1).
More>>This Anti-Dynamin related protein 1 (Drp1) Antibody is validated for use in WB, IC for the detection of Dynamin related protein 1 (Drp1). Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Dynamin family member proline-rich carboxyl-terminal domain less
Dymple
Dynamin-related protein 1
Background Information
Dynamin related protein 1 (Drp1), also known as Dynamin-like protein 1, mediates outter mitochondrial membrane fission in mammilian cells. Like other members of the Dynamin superfamily of GTPases, Drp1 contains a N-terminal tripartate GTP-binding domain and exists as a T-shaped dimer with a head, leg, and stalk. The addition of GTP induces head and stalk rearrangement resulting in membrane constriction. Drp1 has been shown to affect mitochondrial morphology and places a significant role in mitochondrial and peroxisomal fission in mammilian cells. During apoptosis, Drp1 translocates from the cytosol to mitochondria and localizes to potential organelle division sites. Studies show Drp1 inhibition averts cell death, reinforcing to the critical role that mitochondrial fission plays in apoptosis.
References
Product Information
Format
Affinity Purified
Control
Mouse embryonic stem cell lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
FUNCTION: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport (By similarity).
CATALYTIC ACTIVITY: GTP + H2O = GDP + phosphate.
SUBUNIT STRUCTURE: Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule By similarity. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria (By similarity).
SUBCELLULAR LOCATION: Cytoplasm › cytosol (By similarity). Golgi apparatus (By similarity). Endomembrane system; Peripheral membrane protein. Note: Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex (By similarity).
TISSUE SPECIFICITY: Expressed in the cerebellum and in several regions of the cerebrum and diencephalon. Strongly expressed in the cerebellar Purkinje cells and in the pontile giant neurons.
DOMAIN: The GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization (By similarity).
PTM: Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-643 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-622 also promotes mitochondrial fission (By similarity).
Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity (By similarity).
DISRUPTION PHENOTYPE: In embryonic fibroblasts, mice show defects in trophoblast giant cells and cardiomyocytes. Extensive mitochondrial networks are formed, and peroxisomes elongated. In brain, there are developmental defects in which Purkinje cells contained few giant mitochondria. In the forebrain, there is a decreased number of neurites and defective synapse formation. Embryos undergo developmentally-regulated apoptosis during neural tube formation. Mice die by around embryonic day 12.
SEQUENCE SIMILARITIES: Belongs to the dynamin family.
Contains 1 GED domain.
Molecular Weight
~80kDa (isoform 2) & ~82 kDa (isoform 1) observed 5 isoforms are produced by alternative splicing: Isoform 1: 80 kDa, Isoform 2: 82 kDa, Isoform 3: 78 kDa, Isoform 4: 69 kDa, and Isoform 5: 24 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse embryonic stem cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Dynamin related protein 1 (Drp1) on 10 µg of mouse embryonic stem cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
ABT155
04053252427145
Documentation
Anti-Dynamin related protein 1 (Drp1) Antibody SDB
