Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Detect ganglioside GD3 using this mouse monoclonal Anti-Diasialoganglioside GD3, clone R24 Antibody, Cat. No. MABC1112, validated for use in Flow Cytometry and Immunocytochemistry, Western Blotting, Affects Function, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry, Affinit
More>>Detect ganglioside GD3 using this mouse monoclonal Anti-Diasialoganglioside GD3, clone R24 Antibody, Cat. No. MABC1112, validated for use in Flow Cytometry and Immunocytochemistry, Western Blotting, Affects Function, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry, Affinit Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Gangliosides are sialic acid-containing oligoglycosylceramides. They are classified based on the number of sialic acid residues per molecule. GM gangliosides contain one sialic acid residue, GD gangliosides contain two, GT contain three, and GQ contain four sialic acid residues. Sialyltransferase-catalyzed 2-3 sialylation of the galactose of the trisaccharide Gal 1-4Glc 1-1 Cer produces GM3, which can be further sialylated to produce GD3 with two sialic acid on the galactose. GD3 is found in melanocytes and other cells that originate in the neuroectoderm. It is expressed almost exclusively among gangliosides in the early stage of the brain development of mice and rats. It is also detected in the T cell malignant leukocytes, such as acute lymphoblastic leukemia cells, adult T cell leukemia cells, and in activated normal human T lymphocytes. GD3 is found in almost all melanomas and is considered as an important biomarker for malignant melanoma. GD3 positive cells show markedly increased cell growth and invasive characteristics. Suppression of GD3 expression with antisense GD3 synthase cDNA is shown to reduce tumor cell phenotypes such as growth, migration, metastasis, and angiogenesis in F11 cells. Intra-cytoplasmic domain of neogenin is reported to act as a driver to promote various cancer phenotypes. Ref.: Hamamura, K et al. (2005). Proc. Natl. Acad. Sci. USA. 102 (31), 11041 11046; Dippold, WG et al. (1985). Cancer Res. 45(8): 3699-3705; Kaneko, K et al (2016). J. Biol. Chem. (In press).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG3 in PBS without azide.
Detect ganglioside GD3 using this mouse monoclonal Anti-Diasialoganglioside GD3, clone R24 Antibody, Cat. No. MABC1112, validated for use in Flow Cytometry and Immunocytochemistry, Western Blotting, Affects Function, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry, Affinit
Key Applications
Affects Function
Flow Cytometry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Affinity Binding Assay
Application Notes
Affects Function: A representative lot induced cellular Lyn kinase activity and tyrosine phosphorylation of cellular proteins of primary rat cerebellar cultures in a time-dependent manner (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).
Affinity Binding Assay: A representative lot reacted with heat stable glycolipid on the surface of all SK-MEL melanoma and two of the five astrocytoma cells tested, whereas epithelial cell types, fibroblasts, and cells of hematopoietic origin were found to lack the surface antigen recognized by clone R24 (Dippold, W.G., et al. (1980). Proc. Natl. Acad. Sci. U.S.A. 77(10):6114-6118).
Flow Cytometry Analysis: A representative lot immunostained the surface of cultured primary rat cerebellar cells (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).
Immunocytochemistry Analysis: A representative lot immunostained NGF-induced nurite outgrowths of cultured DRG neurons from wild-type, but not GD3 synthase-knockout, mice (Ribeiro-Resende, V.T., et al. (2014). PLoS One. 9(10):e108919).
Immunohistochemistry Analysis: A representative lot detected GD3 immunoreactivity in unfixed frozen tissue samples of primary melanoma and metastatic malignant melanoma (Dippold, W.G., et al. (1985). Cancer Res. 45(8):3699-3705).
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated Lyn from rat brain membrane extract. Clone R24 co-immunoprecipitated caveolin and the exogenously expressed Lyn, but not Src, from CHO cells expressing exogenous GD3 synthase (Kasahara, K., et al. (1997). J. Biol. Chem. 272(47):29947-29953).
Biological Information
Immunogen
SK-MEL-28 human melanoma cells (Dippold, W.G., et al. (1980). Proc. Natl. Acad. Sci. U.S.A. 77(10):6114-6118).
Clone
R24
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone R24 (a.k.a. R-24) immunostained neurites of cultured DRG neurons from wild-type, but not GD3 synthase-knockout, mice (Ribeiro-Resende, V.T., et al. (2014). PLoS One. 9(10):e108919).
Isotype
IgG3κ
Species Reactivity
Human
Rat
Mouse
Species Reactivity Note
Target glycolipid is not species-specific.
Antibody Type
Monoclonal Antibody
Purification Method
Protein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Flow Cytometry Analysis in SK-MEL-28 human skin melanoma cells.
Flow Cytometry Analysis: 0.2 µL of this antibody detected GD3 immunoreactivity on the surface of one million SK-MEL-28 human skin melanoma cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
