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ST1703 Anti-DLL1 Mouse mAb (4F8)

Anti-DLL1, mouse monoclonal, clone 4F8, recognizes the DLL1 protein in HeLa cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.

Anti-DLL1 Mouse mAb (4F8): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Delta-like 1

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ST1703
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the DLL1 protein in HeLa cells.
      Catalogue NumberST1703
      Brand Family Calbiochem®
      SynonymsAnti-Delta-like 1
      Application Data
      Detection of human DLL1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human DLL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µ/g/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 18-110 human DLL1, expressed as a GST fusion protein
      Clone4F8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1703 0

      Documentation

      Anti-DLL1 Mouse mAb (4F8) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-July-2012 JSW
      SynonymsAnti-Delta-like 1
      ApplicationELISA (see coments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human DLL1 by immunoblotting. Sample: Partial recombinant protein. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human DLL1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-DLL1 Mouse mAb (4F8) (Cat. No. ST1703) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the DLL1 protein.
      BackgroundThe DLL1 protein is a human homolog of the Notch Δ ligand and is a member of the δ/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may also play a role in cell-to-cell communication.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 18-110 human DLL1, expressed as a GST fusion protein
      Clone4F8
      IsotypeIgG2a
      Specieshuman
      Positive controlHeLa cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µ/g/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review