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ABE374 Anti-DAZ Antibody

Anti-DAZ Antibody is a Rabbit Polyclonal Antibody for detection of DAZ also known as Deleted in azoospermia protein 1 & has been validated in WB, IHC(P).

Anti-DAZ Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: RNA Metabolism & Binding Proteins Gene Symbol: DAZ1, DAZ, SPGY UniProt Number: Q9NQZ3
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ABE374
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Chp, Eq, B, Rhesus Macaque, PoWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE374
      DescriptionAnti-DAZ Antibody
      Alternate Names
      • Deleted in azoospermia protein 1
      Background InformationDAZ is an RNA-binding protein that belongs to the DAZ family of proteins required for normal growth, development, and fertility. The DAZ family of proteins have one or more RNA recognition motif (RRM) domains and multiple DAZ repeats. Mammalian DAZ is widely expressed in adults with high levels of expression in testis. Frequent deletion of the DAZ genes suggests that DAZ plays an important role in spermatogenesis. It was even proposed that DAZ family proteins are involved in mRNA metabolism during spermatogenesis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Human testis tissue lysate.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-DAZ Antibody is a Rabbit Polyclonal Antibody for detection of DAZ also known as Deleted in azoospermia protein 1 & has been validated in WB, IHC(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:400 dilution from a representative lot detected DAZ in human testis tissue.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human DAZ.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes the N-terminus of DAZ. This antibody demonstrates cross-reactivity with DAZ2, DAZ3, DAZ4, and DAZL each of which contains repeats of the DAZ gene.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Chimpanzee
      • Horse
      • Bovine
      • Rhesus Macaque
      • Pig
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat.
      Predicted to react with Chimpanzee, Equine, Bovine, Rhesus Macaque, and Porcine.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a member of the DAZ gene family and is a candidate for the human Y-chromosomal azoospermia factor (AZF). Its expression is restricted to premeiotic germ cells, particularly in spermatogonia. It encodes an RNA-binding protein that is important for spermatogenesis. Four copies of this gene are found on chromosome Y within palindromic duplications; one pair of genes is part of the P2 palindrome and the second pair is part of the P1 palindrome. Each gene contains a 2.4 kb repeat including a 72-bp exon, called the DAZ repeat; the number of DAZ repeats is variable and there are several variations in the sequence of the DAZ repeat. Each copy of the gene also contains a 10.8 kb region that may be amplified; this region includes five exons that encode an RNA recognition motif (RRM) domain. This gene contains three copies of the 10.8 kb repeat. However, no transcripts containing three copies of the RRM domain have been described; thus the RefSeq for this gene contains only two RRM domains.
      Gene Symbol
      • DAZ1
      • DAZ
      • SPGY
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: RNA-binding protein that plays an essential role in spermatogenesis. May act by binding to the 3'-UTR of mRNAs and regulating their translation. Promotes germ-cell progression to meiosis and formation of haploid germ cells.

      SUBUNIT STRUCTURE: Forms a heterodimer with BOLL and DAZL. Interacts with PUM2, DAZAP1, DAZAP2, DZIP1 and DZIP3.

      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Predominantly cytoplasmic. Nuclear at some stages of spermatozoide development. Localizes both to the nuclei and cytoplasm of spermatozoide differentiation. Nuclear in fetal gonocytes and in spermatogonial nuclei. It then relocates to the cytoplasm during male meiosis.

      TISSUE SPECIFICITY: Testis-specific. Expression restricted to premeiotic germ cells, particularly in spermatogonia (at protein level).
      Molecular Weight~83 kDa calculated. Additional bands due to polymorphic expression of DAZ as well as cross-reactivity with DAZ2, DAZ3, DAZ4, and DAZL with calculated molecular weights of ~33 kDa, 37 kDa, 75 kDa, and 100 kDa may be observed in some cell lysates (Poongothai, J., et al. (2009). Singapore Med J. 50(4):336-347; Kim, B., et al. (2009). Hum Reprod. 24(6):1507-1515). This antibody detects DAZ proteins at ~36 kDa, ~80 kDa, and ~105 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human testis tissue lysate.

      Western Blot Analysis: 1 µg/mL dilution of this antibody detected DAZ in 10 µg of human testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE374 04053252427107