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ABT1493-AF488 Anti-Cofilin-1 Antibody, Alexa Fluor® 488 Conjugate

This rabbit polyclonal Anti-Cofilin-1 Antibody, Alexa Fluor® 488 Conjugate, Cat. No. ABT1493-AF488 is validated for use in Immunocytochemistry for the detection of cofilin-1.

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Research Category: Cell Structure Gene Symbol: CFL, CFL1 UniProt Number: P23528
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ABT1493-AF488
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HICCRbAlexaFluor®488Polyclonal Antibody
      Description
      Catalogue NumberABT1493-AF488
      DescriptionAnti-Cofilin-1 Antibody, Alexa Fluor® 488 Conjugate
      Alternate Names
      • Cofilin-1
      • 18 kDa phosphoprotein
      • Cofilin
      • non-muscle isoform
      • p18
      Background InformationCofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.
      References
      Product Information
      FormatAlexaFluor®488
      PresentationPurified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-Cofilin-1 Antibody, Alexa Fluor® 488 Conjugate, Cat. No. ABT1493-AF488 is validated for use in Immunocytochemistry for the detection of cofilin-1.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cofilin-1 in NIH/3T3 cells.

      The unconjugated antibody (Cat. No. ABT1493) is also available for immunocytochemistry, immunoprecipitation, and Western blotting applications.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis Alexa Fluor® 488-conjugated polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Predicted to react with Bovine, Porcine, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CFL
      • CFL1
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cofilin-1 in HeLa cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABT1493-AF488 04054839086175