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AB5416P Anti-Clock Antibody

Anti-Clock Antibody detects level of Clock & has been published & validated for use in ELISA & WB.

Anti-Clock Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Research Sub-Category: Circadian Rhythm & Sleep Gene Symbol: CLOCK, KAT13D, hCLOCK, KIAA0334, clock UniProt Number: O15516
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AB5416P
50 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5416P
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Clock Antibody
      Alternate Names
      • KAT13D
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity purified immunoglobulin. Liquid in PBS and 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Clock Antibody detects level of Clock & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using ECL.

      ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenAn 18 amino acid peptide mapping downstream of the serine rich region of mouse Clock (Antoch et al. 1997; King et al. 1997).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes mouse Clock. The immunogen shows no significant sequence homology with dPER or other known proteins.

      SPECIES REACTIVITIES: The immunogen sequence is 92% conserved in rate and 90% in human. Reactivity with other species has not been confirmed.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
      Gene Symbol
      • CLOCK
      • KAT13D
      • hCLOCK
      • KIAA0334
      • clock
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15516 # BMAL1/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins.
      SIZE: 846 amino acids; 95304 Da
      SUBUNIT: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with BMAL1 is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and BMAL1. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-BMAL1 heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNT and ARNT2 to form heterodimers which bind poorly to the E-box motif (By similarity).
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus (By similarity).
      TISSUE SPECIFICITY: Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN).
      PTM: Phosphorylation is dependent on CLOCK-BMAL1 heterodimer formation (By similarity).
      SIMILARITY: SwissProt: O15516 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains.
      MISCELLANEOUS: CLOCK-BMAL1 double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

      During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB5416P 04053252330254