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MABS1118 Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1

Detect Cdc4 (FBXW7) using this mouse monoclonal Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1, Cat. No. MABS1118, validated for use in Western Blotting.

Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Gene Symbol: FBXW7, AGO, CDC4, FBW7, FBX30, SEL10 UniProt Number: Q969H0
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MABS1118
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1118
      DescriptionAnti-Cdc4 (FBXW7) Antibody, clone 9D8.1
      Alternate Names
      • F-box/WD repeat-containing protein 7
      • Archipelago
      • F-box and WD-40 domain protein 7
      • F-box protein FBW7
      • F-box protein FBX30
      • F-box protein SEL-10
      • hAgo
      • hCdc4
      • Homolog of C elegans sel-10
      Background InformationF-box/WD repeat-containing protein 7 (UniProt Q969H0; also known as Archipelago, F-box and WD-40 domain protein 7, F-box protein FBW7, F-box protein FBX30, F-box protein SEL-10, hAgo, hCdc4, Homolog of C elegans sel-10) is encoded by the FBXW7 (also known as AGO, CDC4, FBW7, FBX30, SEL10) gene (Gene ID 55294) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. The F-box/WD repeat-containing protein 7 functions as an adaptor component of the Skp1, Cullin, F-box (SCF) and mediates substrates recruitment to the Cullin-RING E3 ubiquitin ligase (CRL) complex. Cyclin E, c-Myc, c-Jun, Notch1, SREBP, mTOR, c-Myb, MCL1, NF B2, and GATA3 are all known substrates targeted by Fbxw7 for ubiquitin-proteasome system- (UPS-) mediated degradation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Cdc4 (FBXW7) using this mouse monoclonal Anti-Cdc4 (FBXW7) Antibody, clone 9D8.1, Cat. No. MABS1118, validated for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:1,000 dilution from a representative lot detected Cdc4 (FBXW7) in 10 µg of NIH/3T3, HeLa, HepG2, K562, LNCaP, U2OS nuclear extracts.
      Biological Information
      ImmunogenGST-tagged recombinant human Cdc4 (FBXW7) isoform 1 (Archipelago alpha, FBW7alpha) N-terminal fragment.
      Clone9D8.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 9D8.1 epitope lies within the N-terminal region specific to human and murine Cdc4 (FBXW7) spliced isoform 1 (Archipelago alpha, FBW7alpha, 110K, common), and not conserved in human/mouse isoform 2 (Archipelago beta, FBW7beta, 69K) or 3 (Archipelago gamma, FBW7gamma, Hippocampal). Note: UniProt Q8VBV4 currently lists only murine spliced isoform 3 (NM_080428), but not isoform 1 (NM_001177774) or 2 (NM_001177773).
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Specific ActivityN-terminal region.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FBXW7
      • AGO
      • CDC4
      • FBW7
      • FBX30
      • SEL10
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~80 kDa observed. 79.66 kDa (human; UniProt Q969H0-1) and mouse; NP_001171244 & NM_001177774) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 nuclear extract.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Cdc4 (FBXW7) in 10 µg of HEK293 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS1118 04054839081354