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IM16 Anti-Cathepsin D (Ab-2) Rabbit pAb

Anti-Cathepsin D (Ab-2) Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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IM16
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~52 kDa latent form D and the ~34 kDa subunit, but not the ~14 kDa subunit of cathepsin D in breast carcinoma tissue extracts.
      Catalogue NumberIM16
      Brand Family Calbiochem®
      References
      ReferencesRochefort, H. 1992. Eur. J. Cancer 28A, 1780.
      Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
      Westly, B. and Rochefort, H. 1980. Cell 20, 352.
      Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlHT1080 concentrated conditioned medium or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (5 µg/ml)
      Immunoblotting (2.5 µg/ml)
      Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
      Application CommentsFor optimal results in immunoblotting, conditioned media from HT1080 cells should be concentrated 10-20X prior to loading. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified human liver cathepsin D
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      IM16 0

      Documentation

      Anti-Cathepsin D (Ab-2) Rabbit pAb SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Cathepsin D (Ab-2) Rabbit pAb Analysenzertifikate

      TitelChargennummer
      IM16

      Literatur

      Übersicht
      Rochefort, H. 1992. Eur. J. Cancer 28A, 1780.
      Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
      Westly, B. and Rochefort, H. 1980. Cell 20, 352.
      Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.

      Broschüre

      Titel
      Cathepsins and Related Products Technical Bulletin
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      ApplicationFrozen Sections (5 µg/ml)
      Immunoblotting (2.5 µg/ml)
      Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~52 kDa latent form and the ~34 kDa (but not the ~14 kDa) active subunit of cathepsin D.
      BackgroundCathepsin D (CD) is a normal lysosomal proteinase expressed in all cells. It is an aspartyl proteinase, with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. CD is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid pro fragment and an internal cleavage to generate the two chain form made up of 34 and 14 kDa subunits. It contains the mannose-6-phosphate lysosomal localization signal which targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, CD is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that CD plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
      HostRabbit
      Immunogen speciesHuman
      Immunogenpurified human liver cathepsin D
      IsotypeIgG
      Specieshuman
      Positive controlHT1080 concentrated conditioned medium or breast carcinoma tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor optimal results in immunoblotting, conditioned media from HT1080 cells should be concentrated 10-20X prior to loading. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRochefort, H. 1992. Eur. J. Cancer 28A, 1780.
      Rochefort, H. 1990. In Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 153.
      Westly, B. and Rochefort, H. 1980. Cell 20, 352.
      Barret, A. J. 1977. In Proteinases in Mammalian Cells and Tissues, ed. Barret, A. J., North Holland, New York, p 209.