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Anti-CXCL1/GROa, clone HL2401, Cat. No. MABF2761, is a mouse monoclonal antibody that detects CXCL1 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.
More>>Anti-CXCL1/GROa, clone HL2401, Cat. No. MABF2761, is a mouse monoclonal antibody that detects CXCL1 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting. Less<<
Growth-regulated alpha protein (UniProt: P09341; also known as C-X-C motif chemokine 1, CXCL1, GRO-alpha(1-73), Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3) is encoded by the CXCL1 (also known as GRO, GRO1, GROA, MGSA, SCYB1) gene (Gene ID: 2919) in human. CXCL1 is a multi-functional chemokine that displays chemotactic activity for neutrophils. It is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature protein that can be further cleaved into three chains (aa 4-73; 5-73; and 6-73) via proteolytic cleavage after secretion from peripheral blood monocytes. CXCL1 may play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. Its levels are reported to be low under basal conditions but increase significantly following active infection. It plays a role in recruiting and activating neutrophils for microbial killing at the tissue site and can also activate the release of proteases and reactive oxygen species. CXCL1 signals through binding to CXCR2, a G-protein coupled receptor. Aberrant expression of CXCL1 has been reported in several cancers and has been associated with oncogenesis, metastasis, angiogenesis and chemoresistance. Clone HL2401 is shown to specifically binds to CXCL1, although it may show slight binding to CXCL2. It is shown to reduce both cellular proliferation and invasion of cancer cells overexpressing CXCL1. It also induces apoptosis and impairs tumor vasculature. (Ref.: Miyake, M., et al. (2019). Theranostics.;9(3):853-867; Yang C., et al. (2019). Int. J. Oncol. 55(3); 684-696Wuyts, A., et al. (1999). Eur. J. Biochem. 260(2); 421-429).
References
Product Information
Format
Purified
Control
MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
000+ and ever-growing portfolio of antibodies to meet your research needs in Apoptosis and cancer
Cell structure
Cell signaling
Epigenetics
Inflammation
Immunology
Neuroscience
Stem cell biology
and more. Visit our Antibody Explorer Tool to view antibodies by clonality
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Presentation
Purified mouse monoclonal antibody IgG1 in PBS with 0.02% sodium azide.
Anti-CXCL1/GROa, clone HL2401, Cat. No. MABF2761, is a mouse monoclonal antibody that detects CXCL1 and is tested for use in ELISA, Immunoprecipitation, Inhibition Assay, and Western Blotting.
Key Applications
Western Blotting
Immunoprecipitation
Inhibition
ELISA
Application Notes
Tested applications
ELISA Analysis: A representative lot detected CXCL1 in ELISA application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).
Inhibition Assay: A representative lot inhibited proliferation and invasion of bladder and prostate cells and disrupted endothelial sprouting in vitro. (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).
Immunoprecipitation Analysis: A representative lot immunoprecipitated CXCL1 in Immunoprecipitation application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).
Western Blotting Analysis: A representative lot detected CXCL1 in Western Blotting application (Miyake, M., et al. (2019). Theranostics. 9(3):853-867).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
Native human CXCL1 protein.
Epitope
Unknown
Clone
HL2401
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone HL2401 is a mouse monoclonal antibody that detects CXCL1.
~15 kDa observed; 11.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting with recombinant Human CXCL1 protein.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant Human CXCL1 protein.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
