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ABT10 Anti-CKAP5 Antibody

Anti-CKAP5 Antibody is an antibody against CKAP5 for use in IC.

Anti-CKAP5 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: CKAP5 UniProt Number: Q14008
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ABT10
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, B, CaICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT10
      DescriptionAnti-CKAP5 Antibody
      Alternate Names
      • Cytoskeleton-associated protein 5
      • Colonic and hepatic tumor over-expressed gene protein
      • Ch-TOG
      Background InformationCytoskeleton-associated protein 5 (CKAP5) is a member of the TOG/XMAP215 family containing heat repeats and plays a role in kinetochore mircotubule protection from depolymerization by MCAK. CKAP5 is the homolog of the Xenopus protein, XMAP25 (also known as chTog), which plays an essential role in centrosomal assembly. CKAP5 is localized in the perinuclear cytoplasm during interphase and at the spindle poles and centrosomes during mitosis. Studies show CKAP5 interacts with TACC1, TACC3, and tubulin leading to the hypothesis that it functions in spindle microtuble organization. Hepatomas and colonic tumors have shown overexpression of CKAP5.
      References
      Product Information
      FormatAffinity Purified
      Control
      • A431 cells
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CKAP5 Antibody is an antibody against CKAP5 for use in IC.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:250 dilution from a previous lot detected CKAP5 in RPE1 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human CKAP5.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Bovine
      • Canine
      Species Reactivity NoteDemonstrated to react with Human.
      Predicted to react with Mouse, Rat, Bovine, and Canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation; it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for directional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interacting with heterogeneous nuclear ribonucleoprotein (hnRNP) A2, which associates with MBP. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011].
      Gene Symbol
      • CKAP5
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Plays a major role in organizing spindle poles.

      SUBUNIT STRUCTURE: Interacts with TACC1.

      TISSUE SPECIFICITY: Overexpressed in hepatomas and colonic tumors. Also expressed in skeletal muscle, brain, heart, placenta, lung, liver, kidney and pancreas.

      SEQUENCE SIMILARITIES: Belongs to the TOG/XMAP215 family.

      Contains 10 HEAT repeats.

      SEQUENCE CAUTION: The sequence AAH17856.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

      The sequence AAH70136.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

      The sequence AAI11044.1 differs from that shown. Reason: Contaminating sequence. Potential poly-A sequence.

      The sequence AAI20871.1 differs from that shown. Reason: Frameshift at position 18.

      The sequence BAA07892.2 differs from that shown. Reason: Erroneous initiation.
      Molecular Weight226 kDa calculated.
      Three isoforms are produced by alternative splicing with molecular weights of 225, 218, and 226 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected CKAP5 in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABT10 04053252633973