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OP60 Anti-Bcl-2 (Ab-1) Mouse mAb (100)

Anti-Bcl-2 (Ab-1) Mouse mAb (100): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Bcl-2α

OP60
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~24-26 kDa Bcl-2 protein in MCF-7 cells, tonsil, and lymph node tissue.
      Catalogue NumberOP60
      Brand Family Calbiochem®
      SynonymsAnti-Bcl-2α
      References
      ReferencesKozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Silverman, G.A., 1993. Mol. Cell. Biol. 13, 5469.
      Vaux, D.L. 1993. Proc. Natl. Acad. Sci. USA 90, 786
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
      Larsen, C.J., et al. Nouv. Rev. Fr. Hematol. 35, 37.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlMCF7 cells or tonsil or lymph node tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Pezzella, F., et al. 1990. Am. J. Pathol. 137, 225.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, see comments)
      Application CommentsGenerally, no pre-treatment of paraffin sections is needed, but treating with heat may improve staining in some tissues. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogena synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 of human Bcl-2
      ImmunogenHuman
      Clone100
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      OP60 0

      Documentation

      Anti-Bcl-2 (Ab-1) Mouse mAb (100) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Bcl-2 (Ab-1) Mouse mAb (100) Analysenzertifikate

      TitelChargennummer
      OP60

      Literatur

      Übersicht
      Kozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Silverman, G.A., 1993. Mol. Cell. Biol. 13, 5469.
      Vaux, D.L. 1993. Proc. Natl. Acad. Sci. USA 90, 786
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
      Larsen, C.J., et al. Nouv. Rev. Fr. Hematol. 35, 37.

      Broschüre

      Titel
      Caspases and other Apoptosis Related Tools Brochure
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-October-2007 RFH
      SynonymsAnti-Bcl-2α
      ApplicationFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (1-10 µg/ml, see comments)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-AG14 plasmacytoma cells (see application references). Recognizes the ~24-26 kDa bcl-2 protein.
      BackgroundThe bcl-2 proto-oncogene is encoded by a gene 230 kb in size that gives rise to a ~24-26 kDa protein which has been localized to the mitochondrial membrane. Although some data exists for implicating bcl-2 as a novel GTP binding protein, the major function of bcl-2 appears to be an inhibitor of apoptosis or "programmed cell death". It has now been well documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore constitutively activated in B cell malignancies (i.e. follicular lymphoma and chronic lymphocytic leukemia). In addition, bcl-2 has also been shown to protect mammalian cells from sodium azide, colchicine, DNA, RNA, and protein-synthesis inhibitors, as well as steroids, heat shock and irradiation.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic peptide (GAAPAPGIFSSQPG) corresponding to amino acids 41-54 of human Bcl-2
      Clone100
      IsotypeIgG₁
      Specieshuman, not mouse
      Positive controlMCF7 cells or tonsil or lymph node tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsGenerally, no pre-treatment of paraffin sections is needed, but treating with heat may improve staining in some tissues. Antibody should be titrated for optimal results in individual sample types.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesKozopas, K.M., et al. 1993. Proc. Natl. Acad. Sci. USA 90, 3516.
      Silverman, G.A., 1993. Mol. Cell. Biol. 13, 5469.
      Vaux, D.L. 1993. Proc. Natl. Acad. Sci. USA 90, 786
      Wagner, A.J., et al. 1993. Mol. Cell. Biol. 13, 2432.
      Hockenberry, D.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 6961.
      Reed, J.C., et al. 1991. Exp. Cell. Res. 195, 277.
      Reed, J.C., et al. 1990. Mol. Cell. Biol. 10, 4370
      Nunez, G., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 4589.
      Larsen, C.J., et al. Nouv. Rev. Fr. Hematol. 35, 37.
      Application referencesOriginal Clone Pezzella, F., et al. 1990. Am. J. Pathol. 137, 225.