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CA1033 Anti-BRCA2 Rabbit pAb

Anti-BRCA2 Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Breast Cancer Susceptibility Protein

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CA1033
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~400 kDa BRCA2 protein in MCF-7 and HeLa cells and breast carcinoma.
      Catalogue NumberCA1033
      Brand Family Calbiochem®
      SynonymsAnti-Breast Cancer Susceptibility Protein
      Application Data
      Detection of human BRCA2 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (750 µg) (lower panel) and HeLa cells (750 µg) (upper panel). Antibody for IP: Rabbit IgG Control (5µg, lane 1) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (5 µg, lane 2; 10 µg, lane 3; and 15 µg, lane 4). Conditions for immunoblotting: Samples: Whole cell lysate (100 µg) from MCF-7 cells and HeLa cells (lane 5, lower and upper panels, respectively) and BRCA2 IP samples (lanes 1-4). Primary antibody: Anti-BRCA2 (Ab-1) Mouse mAb (2B) (Cat. No. OP95) (2 µg/ml). Detection: chemiluminescence.

      Detection of human BRCA2 by staining paraffin sections. Sample: Breast carcinoma tissue (formalin-fixed, paraffin-embedded, heat-pretreated in 10 mM sodium citrate buffer). Primary antibody: Rabbit IgG Control (10 µg/ml, left panel) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (10 µg/ml, right panel). Detection: DAB with methyl green counterstain.
      References
      ReferencesFirst International Workshop on the Function of BRCA1 and BRCA2. 11-12 September, 1997. Churchill College, Cambridge, UK.
      Ludwig, T., et al. 1997. Genes Devel. 11, 122.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Rajan, J.V., et al. 1997. Dev. Biol. 184, 385.
      Sharan, S.K., et al. 1997. Nature 386, 804.
      Suzuki, A., et al. 1997. Genes Devel. 11, 1242.
      Canon-Albright, L.A. and Skolnick, M.H. 1996. Semin. Oncol. 23, 1.
      Goggins, M., et al. 1996. Cancer Res. 56, 5360.
      Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13078.
      Spillman, M.A. and Bowcock, A.M. 1996. Oncogene 13, 1639.
      Stratton, M.R. 1996. Hum. Mol. Genet. 5, 1515.
      Tavtigian, S.V., et al. 1996. Nat. Genet. 12, 333.
      Vaughn, J.P., et al. 1996. Cancer Res. 56, 4590.
      Wooster, R., et al. 1995. Nature 378, 789.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlMCF-7 and HeLa cells and breast carcinoma
      Preservative≤ 0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (2 µg/ml; recombinant protein only)
      Paraffin Sections (10 µg/ml, heat-pretreatment required, see comments)
      Immunoprecipitation (10 µg per 750 µg total protein)
      Application CommentsThis antibody only recognizes recombinant BRCA2 by immunoblotting. For staining paraffin sections, pretreat tissue sections by heating in 10 mM citrate buffer, pH 6.0, for 15 min. Reactivity towards BRCA2 from other species may also occur. BRCA2 protein contains 3418 amino acids with a theoretical isolectric point of 6.3 and a calculated molecular weight of ~376 kDa. However, BRCA2 appears to migrate on SDS/PAGE as a protein with an apparent size of greater than 400 kDa and will co-migrate with the 470 kDa catalytic subunit of DNA-PK. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 3245-3418 of human BRCA2
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      CA1033 0

      Documentation

      Anti-BRCA2 Rabbit pAb SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-BRCA2 Rabbit pAb Analysenzertifikate

      TitelChargennummer
      CA1033

      Literatur

      Übersicht
      First International Workshop on the Function of BRCA1 and BRCA2. 11-12 September, 1997. Churchill College, Cambridge, UK.
      Ludwig, T., et al. 1997. Genes Devel. 11, 122.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Rajan, J.V., et al. 1997. Dev. Biol. 184, 385.
      Sharan, S.K., et al. 1997. Nature 386, 804.
      Suzuki, A., et al. 1997. Genes Devel. 11, 1242.
      Canon-Albright, L.A. and Skolnick, M.H. 1996. Semin. Oncol. 23, 1.
      Goggins, M., et al. 1996. Cancer Res. 56, 5360.
      Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13078.
      Spillman, M.A. and Bowcock, A.M. 1996. Oncogene 13, 1639.
      Stratton, M.R. 1996. Hum. Mol. Genet. 5, 1515.
      Tavtigian, S.V., et al. 1996. Nat. Genet. 12, 333.
      Vaughn, J.P., et al. 1996. Cancer Res. 56, 4590.
      Wooster, R., et al. 1995. Nature 378, 789.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-October-2008 JSW
      SynonymsAnti-Breast Cancer Susceptibility Protein
      ApplicationImmunoblotting (2 µg/ml; recombinant protein only)
      Paraffin Sections (10 µg/ml, heat-pretreatment required, see comments)
      Immunoprecipitation (10 µg per 750 µg total protein)
      Application Data
      Detection of human BRCA2 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (750 µg) (lower panel) and HeLa cells (750 µg) (upper panel). Antibody for IP: Rabbit IgG Control (5µg, lane 1) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (5 µg, lane 2; 10 µg, lane 3; and 15 µg, lane 4). Conditions for immunoblotting: Samples: Whole cell lysate (100 µg) from MCF-7 cells and HeLa cells (lane 5, lower and upper panels, respectively) and BRCA2 IP samples (lanes 1-4). Primary antibody: Anti-BRCA2 (Ab-1) Mouse mAb (2B) (Cat. No. OP95) (2 µg/ml). Detection: chemiluminescence.

      Detection of human BRCA2 by staining paraffin sections. Sample: Breast carcinoma tissue (formalin-fixed, paraffin-embedded, heat-pretreated in 10 mM sodium citrate buffer). Primary antibody: Rabbit IgG Control (10 µg/ml, left panel) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (10 µg/ml, right panel). Detection: DAB with methyl green counterstain.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~400 kDa BRCA2 protein.
      BackgroundInherited mutations in the BRCA2 gene enhances the risk of familial breast cancer with the neoplasia related to loss of the wild type allele. Human BRCA2 encodes a 3418 amino acid protein with little homology to any known proteins. Transcription of BRCA2 appears to be generally co-ordinately regulated and co-locally expressed with BRCA1 during proliferation and differentiation with low level expression in early G1 and maximal expression occurring during the G1/S transition of the cell cycle. However, BRCA1 and BRCA2 expression is differentially regulated during the development of specific endocrine tissues suggesting a gene-specific response to sex hormones. While loss of BRCA2 is associated with early onset breast cancer, the gene is critical for embryogenesis since nullizygous mice with a deletion of the 5' region of exon 11 arrest development by day 8.5 of gestation. Recently, however, mice carrying homozygous inactivating mutations due to deletion of exon 11 sequences or insertion of selectable markers in the 3' portion of the cDNA have been obtained, albeit with severe growth characteristics including lack of germinal cells, small stature, and skeletal deformities. Loss of BRCA2 expression in embryogenesis does not appear to be due to apoptosis, but proliferation is impaired, perhaps due to enhanced expression of p21WAF1. Although the precise role for BRCA2 is still to be determined, recent studies implicate BRCA2 in DNA repair and recombination processes partly due to association with the Rad51 protein.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 3245-3418 of human BRCA2
      IsotypeIgG
      Specieshuman
      Positive controlMCF-7 and HeLa cells and breast carcinoma
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤ 0.1% sodium azide
      CommentsThis antibody only recognizes recombinant BRCA2 by immunoblotting. For staining paraffin sections, pretreat tissue sections by heating in 10 mM citrate buffer, pH 6.0, for 15 min. Reactivity towards BRCA2 from other species may also occur. BRCA2 protein contains 3418 amino acids with a theoretical isolectric point of 6.3 and a calculated molecular weight of ~376 kDa. However, BRCA2 appears to migrate on SDS/PAGE as a protein with an apparent size of greater than 400 kDa and will co-migrate with the 470 kDa catalytic subunit of DNA-PK. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFirst International Workshop on the Function of BRCA1 and BRCA2. 11-12 September, 1997. Churchill College, Cambridge, UK.
      Ludwig, T., et al. 1997. Genes Devel. 11, 122.
      Mizuta, R., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 6927.
      Rajan, J.V., et al. 1997. Dev. Biol. 184, 385.
      Sharan, S.K., et al. 1997. Nature 386, 804.
      Suzuki, A., et al. 1997. Genes Devel. 11, 1242.
      Canon-Albright, L.A. and Skolnick, M.H. 1996. Semin. Oncol. 23, 1.
      Goggins, M., et al. 1996. Cancer Res. 56, 5360.
      Rajan, J.V., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 13078.
      Spillman, M.A. and Bowcock, A.M. 1996. Oncogene 13, 1639.
      Stratton, M.R. 1996. Hum. Mol. Genet. 5, 1515.
      Tavtigian, S.V., et al. 1996. Nat. Genet. 12, 333.
      Vaughn, J.P., et al. 1996. Cancer Res. 56, 4590.
      Wooster, R., et al. 1995. Nature 378, 789.