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Anti-BLAST-2 (CD23), clone EBVCS4, Cat. No. MABF2319, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tesed for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Radioimmunoassay.
More>>Anti-BLAST-2 (CD23), clone EBVCS4, Cat. No. MABF2319, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tesed for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Radioimmunoassay. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABF2319-25UG
Description
Anti-BLAST-2 (CD23) Antibody, clone EBVCS4
Alternate Names
Low affinity immunoglobulin epsilon Fc receptor
C-type lectin domain family 4 member J
CD23
Fc-epsilon-RII
Immunoglobulin E-binding factor
Lymphocyte IgE receptor
Background Information
Low affinity immunoglobulin epsilon Fc receptor (UniProt: P06734; also known as BLAST-2, C-type lectin domain family 4 member J, Fc-epsilon-RII, Immunoglobulin E-binding factor, Lymphocyte IgE receptor, CD23) is encoded by the FCER2 (also known as CD23A, CLEC4J, FCE2, IGEBF) gene (Gene ID: 2208) in human. Epstein-Barr virus (EBV) is reported to transform human B lymphocytes and is a causative agent for Burkitt s lymphoma and infectious mononucleosis. EBV induces the expression of one or more antigens (e.g., CD23) on the surface of adult human B-lymphocytes soon after infection. CD23 is a homotrimeric, single-pass type II membrane protein that serves as a low-affinity receptor for Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21 and plays an essential role in the regulation of IgE production and in the differentiation of B-cells. CD23 can also exist as a soluble excreted form, sCD23 (aa 150-321), which is produced by ADAM10-mediated ectoderm shedding. CD23 has a cytoplasmic domain (aa 1-21), a transmembrane domain (aa 22-47), and an extracellular domain (aa 48-321). Clone EBVCS4 can specifically detect antigens that are present on the surface of EBV-transformed cells. These antigens are also recognized by autochthonous cytotoxic T cells specific for EBV-transformed cells. (Ref.: Kintner, C., and Sugden, B. (1981). Nature. 294(3); 458-460).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgM in PBS without preservatives.
Applications
Application
Anti-BLAST-2 (CD23), clone EBVCS4, Cat. No. MABF2319, is a mouse monoclonal antibody that detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) and is tesed for use in Flow Cytometry, Immunofluorescence, Immunoprecipitation, and Radioimmunoassay.
Key Applications
Flow Cytometry
Immunofluorescence
Immunoprecipitation
Radioimmunoassay
Application Notes
Radioimmunoassay Analysis: A representative lot detected BLAST-2 (CD23) in Radioimmunoassay applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).
Immunofluorescence Analysis: A representative lot detected BLAST-2 (CD23) in Immunofluorescence applications (Kintner, C., et. al. (1981). Nature. 294(5840):458-60).
Immunoprecipitation Analysis: A representative lot immunoprecipitated BLAST-2 (CD23) in Immunoprecipitation applications (Sugden, B., et. al. (1983). J Virol. 46(3):800-7).
Biological Information
Immunogen
EBV-transformed human cells.
Clone
EBVCS4
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Clone EBVCS4 is a mouse monoclonal antibody that specifically detects Low affinity immunoglobulin epsilon Fc receptor (BLAST-2) in human cells.
Flow Cytometry Analysis: 2 µg of this antibody detected BLAST-2 (CD23) in one million Raji cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Characterization of an antigen whose cell surface expression is induced by infection with Epstein-Barr virus. Sugden, B; Metzenberg, S J Virol
46
800-7
1982
Metabolically labeled monoclonal antibodies were used to measure the number of determinants per cell for an Epstein-Barr virus (EBV) cell surface antigen (EBVCS) (C. Kintner and B. Sugden, Nature [London] 294:458-460, 1981) which is expressed on the surface of EBV-transformed cells. The antigenic determinants were present approximately 5 X 10(5) times per in vitro-transformed cell. Immunoprecipitation followed by electrophoresis in polyacrylamide gels containing sodium dodecyl sulfate indicated that four independent monoclonal antibodies to EBVCS recognized a protein of 47,000 daltons. The identification of EBVCS isolated from EBV-transformed cells grown in tunicamycin demonstrated that the antigen when isolated from cells grown without this drug was glycosylated. Finally, preclearing experiments with monoclonal antibodies to EBVCS or to HLA (class I products of the human major histocompatibility locus) and to beta 2-microglobulin indicated that EBVCS is not a major histocompatibility type 1 antigen.
Identification of antigenic determinants unique to the surfaces of cells transformed by Epstein-Barr virus. Kintner, C; Sugden, B Nature
294
458-60
1981
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