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48-602MAG
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ABD43
Sigma-AldrichAnti-Apelin receptor Antibody
Anti-Apelin receptor, Cat. No. ABD43, is a rabbit polyclonal antibody that detects Apelin receptor and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
More>>Anti-Apelin receptor, Cat. No. ABD43, is a rabbit polyclonal antibody that detects Apelin receptor and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. Less<<
Anti-Apelin receptor Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Apelin receptor (UniProt: P35414; also known as Angiotensin receptor-like 1, G-protein coupled receptor APJ, G-protein coupled receptor HG11) is encoded by the APLNR (also known as AGTRL1, APJ) gene (Gene ID: 187) in human. Apelin receptor(AR), a monomeric transmembrane protein, is a class A GPCR that is widely distributed in human. It is also expressed in embryonic and induced pluripotent stem cells. It is activated by its cognate peptide ligand, apelin, and induces a wide variety of effects, including vasoconstriction and dilation, strengthening cardiac muscle contractility, angiogenesis, fluid homeostasis, and energy metabolism. It also plays role in heart morphogenesis and promotes angioblast migration towards embryonic midline. Apelin-AR signaling has cardioprotective effects and administration of apelin-13 to glucose-deprived cultured cardiomyocytes has inhibitory effects on apoptosis. Glutamic acid 20 and aspartic acid 23 at the extracellular N-terminus of AR are reported to be the first physiological sites that bind apelin. After exposure to apelin, the receptor is internalized from the cell surface into an endosomal recycling compartment, from where it is recycled to the cell membrane. (Ref.: Hu, G., et al. (2021). Front. Physiol. 12; 632886; Wang, Z., et al. (2015). Sci. Rep. 5; 8170; Chapman, NA., et al. (2014). Biochem. Cell Biol. 92(6); 431-440).
References
Product Information
Format
Affinity Purified
Control
Mouse Cerebellum Tissue Lysate
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Anti-Apelin receptor, Cat. No. ABD43, is a rabbit polyclonal antibody that detects Apelin receptor and is tested for use in Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting.
Key Applications
Western Blotting
Peptide Inhibition Assay
Immunohistochemistry (Paraffin)
Application Notes
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Apelin receptor in Mouse cerebellum, Human cerebral cortex, and Mouse cerebral cortex tissue sections.
Peptide Inhibition Assay: Target band detection in Mouse cerebellum tissue lysate was prevented by pre-blocking of a representative lot with the immunogen peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 13 amino acids from the third cytoplasmic domain within the C-terminal half of human Apelin receptor.
Epitope
Cytoplasmic domain
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This rabbit polyclonal antibody specifically detects Apelin receptor. It targets an epitope within the third cytoplasmic domain from the C-terminal half.
Species Reactivity
Mouse
Human
Species Reactivity Note
Human, Mouse. Predicted to react with Rhesus Macaque, Rat based on 100% sequence homology.
This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009].
FUNCTION: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia.
TISSUE SPECIFICITY: Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon.
SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 1 family.
Molecular Weight
~56 kDa observed; 42.66 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Mouse cerebellum tissue lysate.
Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Apelin receptor in Mouse cerebellum tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Pharmacological and immunohistochemical characterization of the APJ receptor and its endogenous ligand apelin. Medhurst, Andrew D, et al. J. Neurochem., 84: 1162-72 (2003)
2003
Apelin peptides have recently been identified to be the endogenous ligands for the G protein-coupled receptor APJ. However, little is known about the physiological roles of this ligand-receptor pairing. In the present study we investigated the pharmacology of several apelin analogues at the human recombinant APJ receptor using radioligand binding and functional assays. This has led to the identification of key residues in the apelin peptide required for functional potency and binding affinity through structure-activity studies. In particular, we have identified that replacement of leucine in position 5, or arginine in position 2 and 4 of the C-terminal apelin peptide, apelin-13, resulted in significant changes in pharmacology. We also investigated the detailed localization of pre-proapelin and APJ receptor mRNA in a wide range of human, rat and mouse tissues using quantitative RT-PCR, and carried out a detailed immunohistochemical study of the distribution of the APJ receptor in rat brain and spinal cord. Interestingly, the APJ receptor was not only co-localized in white matter with GFAP in the spinal cord, but was also clearly localized on neurones in the brain, suggesting that this receptor and its peptide may be involved in a wide range of biological process yet to be determined.
