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MABF2196-25UG
Sigma-AldrichAnti-Adenoviral capsid protein VI Antibody, clone 9F10
Anti-Adenoviral capsid protein VI, clone 9F10, Cat. No. MABF2196, is a mouse monoclonal antibody that detects Adenoviral capsid protein VI and is tested for use in Western Blotting and Immunofluorescence.
More>>Anti-Adenoviral capsid protein VI, clone 9F10, Cat. No. MABF2196, is a mouse monoclonal antibody that detects Adenoviral capsid protein VI and is tested for use in Western Blotting and Immunofluorescence. Less<<
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Übersicht
Replacement Information
Description
Catalogue Number
MABF2196-25UG
Description
Anti-Adenoviral capsid protein VI Antibody, clone 9F10
Alternate Names
Adenovirus protein 33K
L4-33K
Splicing Factor 33K
Terminase small subunit
Background Information
Protein 33K (UniProt: P24940; also known as L4-33K, Splicing factor 33K, Terminase small subunit) is encoded by the L4 gene in Human adenovirus C serotype 5 (Human adenovirus 5). Adenoviruses are nonenveloped, double-stranded DNA viruses that are shown to express up to 20 distinct mRNAs from five major late transcription unit (MLTU) regions known as L1-L5. During adenovirus 5 infection, a cascade of gene expression results in the production of large amounts of the proteins needed to construct progeny virions. The virion of adenovirus contains 13 different polypeptides, which are shown to form an icosahedral capsid surrounding the genome-containing viral core. The capsid protein VI serves as a membrane lytic factor of adenovirus. In its unprocessed form it is involved in virus assembly and translocation of hexon into the nucleus. The matured capsid protein VI processed by a virion-incorporated adenoviral proteinase at its N and C-terminals. The cleaved C-terminal peptide is shown to act as a coactivator of the viral protease whereas the N-terminal region forms an amphipathic helix that is capable of membrane association and lysis. Clone 9F10 recognizes both the mature and precursor form of capsid protein VI. (Ref.: Martinez, R., et al., (2015). J. Virol. 89(4); 2121-2135; Morris, SJ., et al. (2010). J. Virol. 84(14): 7096-7104).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% sodium azide, pH7.4 with 0.05% sodium azide.
Applications
Application
Anti-Adenoviral capsid protein VI, clone 9F10, Cat. No. MABF2196, is a mouse monoclonal antibody that detects Adenoviral capsid protein VI and is tested for use in Western Blotting and Immunofluorescence.
Key Applications
Western Blotting
Immunofluorescence
Application Notes
Immunofluorescence Analysis: A representative lot detected Adenoviral capsid protein VI in Immunofluorescence applications (Martinez, R., et. al. (2015). J Virol. 89(4):2121-35).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
Recombinant purified adenovirus capsid protein VI.
Clone
9F10
Concentration
1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone 9F10 is a mouse monoclonal antibody that detects adenoviral capsid protein VI.
~31 kDa observed; 25.16 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in U2OS cells transfected with Adenovirus 5.
Western Blotting Analysis: 2 µg/mL of this antibody detected Adenoviral capsid protein VI in U2OS cells transfected with Adenovirus 5.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
MABF2196-25UG
04061841990178
Documentation
Anti-Adenoviral capsid protein VI Antibody, clone 9F10 SDB
Nuclear delivery of the adenoviral genome requires that the capsid cross the limiting membrane of the endocytic compartment and traverse the cytosol to reach the nucleus. This endosomal escape is initiated upon internalization and involves a highly coordinated process of partial disassembly of the entering capsid to release the membrane lytic internal capsid protein VI. Using wild-type and protein VI-mutated human adenovirus serotype 5 (HAdV-C5), we show that capsid stability and membrane rupture are major determinants of entry-related sorting of incoming adenovirus virions. Furthermore, by using electron cryomicroscopy, as well as penton- and protein VI-specific antibodies, we show that the amphipathic helix of protein VI contributes to capsid stability by preventing premature disassembly and deployment of pentons and protein VI. Thus, the helix has a dual function in maintaining the metastable state of the capsid by preventing premature disassembly and mediating efficient membrane lysis to evade lysosomal targeting. Based on these findings and structural data from cryo-electron microscopy, we suggest a refined disassembly mechanism upon entry.
