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MABS830-25UG Anti-Acetyl CoA Carboxylase 1 Antibody, clone 7H4.2

Anti-Acetyl CoA Carboxylase 1 Antibody, clone 7H4.2, Cat. No. MABS830, is a mouse monoclonal antibody that targets Acetyl CoA Carboxylase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

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Research Category: Signaling Gene Symbol: ACACA, ACAC, ACC1, ACCA UniProt Number: Q13085
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MABS830-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS830-25UG
      DescriptionAnti-Acetyl CoA Carboxylase 1 Antibody, clone 7H4.2
      Alternate Names
      • ACC1
      • ACC-alpha
      Background InformationAcetyl-CoA carboxylase 1 (UniProt: Q13085; also known as ACC1, ACC-alpha) is encoded by the ACACA (also known as ACAC, ACC1, ACCA) gene (Gene ID: 31) in human. It catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. ACC1 is a multi-subunit, biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. Two isoforms fof ACC have been reported, alpha and beta that are encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. ACC1 levels are regulated under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. ACC1 activity is inhibited upon phosphorylation, which occurs when glucagon or epinephrine binds to the receptors or when the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acids inhibits the activities of ACC1. ACC is considered as an important target in the design of new anti-obesity drugs.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Acetyl CoA Carboxylase 1 Antibody, clone 7H4.2, Cat. No. MABS830, is a mouse monoclonal antibody that targets Acetyl CoA Carboxylase 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Acetyl CoA Carboxylase 1 in human tonsil, human testis, and human cervical cancer tissue.
      Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Acetyl CoA Carboxylase 1 in 10 µg of NIH/3T3 cell lyate.
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 86 amino acids from the C-terminal end of human Acetyl-CoA carboxylase 1.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7H4.2 detects acetyl-CoA carboxylase 1 in human. It targets an epitope within 86 amino acids from the C-terminal end.
      IsotypeIgG2b
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ACACA
      • ACAC
      • ACC1
      • ACCA
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~265 kDa observed; 265.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.
      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Acetyl CoA Carboxylase 1 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS830-25UG 04054839543722