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Anti-Acetyl -Catenin (Lys49), clone mAb-K49-Ac, Cat. No. MABE1829, is a rat monoclonal antibody that targets Catenin -1 acetylated on Lysine 49 and is tested in Dot Blot, Immunoprecipitation, and Western Blotting.
More>>Anti-Acetyl -Catenin (Lys49), clone mAb-K49-Ac, Cat. No. MABE1829, is a rat monoclonal antibody that targets Catenin -1 acetylated on Lysine 49 and is tested in Dot Blot, Immunoprecipitation, and Western Blotting. Less<<
Catenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on lysine 49 by Ezh2 methyltransferase and acetylated by CBP. The trimethylated form represses neuronal differentiation genes Sox1 and Sox3. The K49 acetylated form acts as a transcriptional co-activator of the key mesodermal differentiation gene t-brachyury (t-bra). K49A mutant form of beta-catenin is shown cause defects in ESC differentiation. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824; Levy, L., et al. (2004). Mol. Cell Biol. 24(8); 3404-3414).
References
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Anti-Acetyl -Catenin (Lys49), clone mAb-K49-Ac, Cat. No. MABE1829, is a rat monoclonal antibody that targets Catenin -1 acetylated on Lysine 49 and is tested in Dot Blot, Immunoprecipitation, and Western Blotting.
Key Applications
Dot Blot
Immunoprecipitation
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected Acetyl a-Catenin (Lys49) in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Dot Blot Analysis: A representative lot detected Acetyl -Catenin (Lys49) in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Acetyl -Catenin (Lys49) in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).
Biological Information
Immunogen
BSA-conjugated linear peptide corresponding to 19 amino acids surrounding acetylated lysine 49 from human -catenin.
Clone
mAb-K49-Ac
Concentration
Please refer to lot specific datasheet.
Host
Rat
Specificity
Clone mAb-K49-Ac is a rat monoclonal antibody that detects -Catenin acetylated on lysine 49.
Isotype
IgG2bκ
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Bovine, Canine based on 100% sequence homology.
~90 kDa observed; 85.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in SW 480 cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected Acetylated -Catenin (Lys49) in SW 480 cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trimethylation and Acetylation of β-Catenin at Lysine 49 Represent Key Elements in ESC Pluripotency. Hoffmeyer, K; Junghans, D; Kanzler, B; Kemler, R Cell Rep
18
2815-2824
2016
Wnt/β-catenin signaling is required for embryonic stem cell (ESC) pluripotency by inducing mesodermal differentiation and inhibiting neuronal differentiation; however, how β-catenin counter-regulates these differentiation pathways is unknown. Here, we show that lysine 49 (K49) of β-catenin is trimethylated (β-catMe3) by Ezh2 or acetylated (β-catAc) by Cbp. Significantly, β-catMe3 acts as a transcriptional co-repressor of the neuronal differentiation genes sox1 and sox3, whereas β-catAc acts as a transcriptional co-activator of the key mesodermal differentiation gene t-brachyury (t-bra). Furthermore, β-catMe3 and β-catAc are alternatively enriched on repressed or activated genes, respectively, during ESC and adult stem cell differentiation into neuronal or mesodermal progenitor cell lineages. Importantly, expression of a β-catenin K49A mutant results in major defects in ESC differentiation. We conclude that β-catenin K49 trimethylation and acetylation are key elements in regulating ESC pluripotency and differentiation potential.
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