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PC285 Anti-AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb

Anti-AML1/RHD Domain (Ab-2) (50-177), rabbit polyclonal, recognizes the ~58 kDa AML1 protein in overexpressing cells such as in Jurkat and MOLT-4 cells. It is validated for Western blotting.

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Synonyme: Anti-Acute Myeloid Leukemia 1, Anti-Runx1

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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, MRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~58 kDa AML1 protein in overexpressing cells such as Jurkat and MOLT-4 cells.
      Catalogue NumberPC285
      Brand Family Calbiochem®
      SynonymsAnti-Acute Myeloid Leukemia 1, Anti-Runx1
      References
      ReferencesStrom, D.K., et al. 2000. J. Biol. Chem. 275, 3438.
      Lo Coco, F., et al. 1997. Haematologica 82, 364.
      Mitani, K., 1997. Leukemia 11 (Suppl 3) 503.
      Mitani, K., 1997. Leukemia 11 (Suppl 3) 294.
      Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
      Hiebert, S.W., et al. Mol. Cell Biol. 16, 1349.
      Kurokawa, M., et al. 1996. J. Biol. Chem. 271, 16870.
      Sacchi, N., et al. 1996. Oncogene 12, 437.
      Meyers, S., et al. 1995. Mol. Cell. Biol. 15, 1974.
      Miyoshi, H., et al. 1995. Nuclei Acids Res. 23, 2762.
      Levanon, D., et al. 1994. Genomics 23, 425.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlJurkat or MOLT-4 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Not Frozen Sections
      Not Immunofluorescence
      Not Immunoprecipitation
      Not Paraffin Sections
      Application NotesImmunoblotting (2.5 µg/ml, chemiluminescence)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody preferentially reacts with the runt homology domain (RHD) on AML1. Reactivity with other AML family members is negligible. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 50-177 of human AML1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      PC285 0

      Documentation

      Anti-AML1/RHD Domain (Ab-2) (50-177) Rabbit pAb Analysenzertifikate

      TitelChargennummer
      PC285

      Literatur

      Übersicht
      Strom, D.K., et al. 2000. J. Biol. Chem. 275, 3438.
      Lo Coco, F., et al. 1997. Haematologica 82, 364.
      Mitani, K., 1997. Leukemia 11 (Suppl 3) 503.
      Mitani, K., 1997. Leukemia 11 (Suppl 3) 294.
      Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
      Hiebert, S.W., et al. Mol. Cell Biol. 16, 1349.
      Kurokawa, M., et al. 1996. J. Biol. Chem. 271, 16870.
      Sacchi, N., et al. 1996. Oncogene 12, 437.
      Meyers, S., et al. 1995. Mol. Cell. Biol. 15, 1974.
      Miyoshi, H., et al. 1995. Nuclei Acids Res. 23, 2762.
      Levanon, D., et al. 1994. Genomics 23, 425.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-August-2008 JSW
      SynonymsAnti-Acute Myeloid Leukemia 1, Anti-Runx1
      ApplicationImmunoblotting (2.5 µg/ml, chemiluminescence)
      Frozen Sections (not recommended)
      Immunofluorescence (not recommended)
      Immunoprecipitation (not recommended)
      Paraffin Sections (not recommended)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~58 kDa AML1 protein.
      BackgroundThe family of nuclear transcription factors represented by AML are important transactivators of tissue-specific genes of the hematopoietic lineage and the bone cell lineage. There are currently three different mammalian proteins that are members of this family, AML1, AML2, and AML3. The AML proteins in turn form heterodimers with another protein, CBFbeta and it is this complex which has the transcription factor activity. Within the complex the AML protein provides the DNA binding function. The AML gene was discovered in 1991 by cloning the t(8;21) translocation that is common to AML. Structurally the AML protein contains a transactivation domain at the C-terminus and has homology with the Drosophila Runt gene that is the DNA binding protein of the protein. In leukemias, AML1 undergoes a translocation and becomes fused to MTG8 (ETO) and forms a chimeric protein (83 kDa). This chimera is in turn complexed with the CBFbeta gene product that also has undergone a translocation. In addition to MTG8, AML1 has also been shown to undergo translocation with TEL, and EVI. Splice variants have been reported for AML which include AML1a (30 kDa), AML1b (54 kDa), and AML1c. All forms contain the runt domain but vary in their transactivation ability. Additionally 6 major transcripts have been shown for AML due to the use of 2 promoters and differential use of 3 polyadenylation sites in addition to the splice variants.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 50-177 of human AML1
      IsotypeIgG
      Specieshuman, mouse
      Positive controlJurkat or MOLT-4 cells
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody preferentially reacts with the runt homology domain (RHD) on AML1. Reactivity with other AML family members is negligible. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesStrom, D.K., et al. 2000. J. Biol. Chem. 275, 3438.
      Lo Coco, F., et al. 1997. Haematologica 82, 364.
      Mitani, K., 1997. Leukemia 11 (Suppl 3) 503.
      Mitani, K., 1997. Leukemia 11 (Suppl 3) 294.
      Crute, B.E., et al. 1996. J. Biol. Chem. 271, 26251.
      Hiebert, S.W., et al. Mol. Cell Biol. 16, 1349.
      Kurokawa, M., et al. 1996. J. Biol. Chem. 271, 16870.
      Sacchi, N., et al. 1996. Oncogene 12, 437.
      Meyers, S., et al. 1995. Mol. Cell. Biol. 15, 1974.
      Miyoshi, H., et al. 1995. Nuclei Acids Res. 23, 2762.
      Levanon, D., et al. 1994. Genomics 23, 425.