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ST1049 Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits.

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ST1049
  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, Rb, R, YeastMMonoclonal Antibody
      Description
      OverviewRecognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
      Catalogue NumberST1049
      Brand Family Calbiochem®
      Application Data
      Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
      References
      ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlHuman placental tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.
      Key Applications Enzyme-Linked Immunosorbent Assay
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunofluorescence
      Immunohistochemistry
      Not Immunoprecipitation
      Application NotesImmunoblotting (1:1000, chemiluminescence)
      Immunoprecipitation (not recommended)
      ELISA (see comments)
      Immunofluorescence (see application references)
      Immunocytochemistry (see application references)
      Immunohistochemistry (see application references)
      Frozen Sections (see application references)
      Application CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogendinitrophenylated proteasomes
      ImmunogenHuman
      Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
      CloneMCP231
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rabbit
      • Rat
      • Yeast
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1049 0

      Documentation

      Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) Analysenzertifikate

      TitelChargennummer
      ST1049

      Literatur

      Übersicht
      Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.

      Broschüre

      Titel
      Proteasome/Ubiquitination NF-kB Pathway Brochure
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-November-2008 JSW
      ApplicationImmunoblotting (1:1000, chemiluminescence)
      Immunoprecipitation (not recommended)
      ELISA (see comments)
      Immunofluorescence (see application references)
      Immunocytochemistry (see application references)
      Immunohistochemistry (see application references)
      Frozen Sections (see application references)
      Application Data
      Detection of human 20S Proteasome α1, 2, 3, 5, 6, 7-subunits by 2D PAGE and immunoblotting. Sample: Whole tissue extract from human placental tissue. Primary antibody: Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231) (Cat. No. ST1049) (1:1000). Detection: luminography.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.
      BackgroundProteasomes are large multi-subunit complexes, that are localized in the nucleus and cytosol, and selectively degrade intracellular proteins. They play a major role in the degradation of many proteins involved in cellular processes such as cell cycling, apoptosis, and proliferation. The human proteasome contains two copies of each of seven different α-type and β-type subunits assembled to form a stack of four seven-membered rings. The α-subunits comprise the outer rings while the β-subunits comprise the inner rings. The α-subunits contain information for the binding of regulatory complexes such as PA700 and PA28.
      HostMouse
      Immunogen speciesHuman
      Immunogendinitrophenylated proteasomes
      Epitopewithin the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
      CloneMCP231
      IsotypeIgG₁
      Specieshuman, mouse, rabbit, rat, yeast
      Positive controlHuman placental tissue
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      CommentsImmunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Highly Toxic
      ReferencesKopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA 94, 2939.
      Application referencesImmunohistochemistry/Immunofluorescence Kabashi, E., et al. 2004. J. Neurochem. 89, 1325. Frozen Sections Fratta, P., et al. 2005. Am. J. Pathol. 167, 517. Immunocytochemistry/Immunofluorescence Taylor, D.M., et al. 2005. Cell Stress Chaperones 10, 230.