Amicon® Ultra-4 Zentrifugen-Filtereinheiten
Provide both ultra-fast spin times and ultra-high recoveries
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Provide both ultra-fast spin times and ultra-high recoveries Weniger
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Überblick
Spezifikationen
Bestellinformationen
Documentation
Literatur
| Übersicht | Anwendung |
|---|---|
| Modification of Allergenicity and immunogenicity of formate dehydrogenase by conjugation with linear mono methoxy poly ethylene glycol: Improvement in detoxification of formate in methanol poisoning Muthuvel A., Rajamani R., Senthilvelan M., Manikandan s., Sheeladevi R. Clinica Chimica acta 374 (2006) 122-128 2005 | Protein Purification |
| Simultaneous reduction and alkylation of protein disulfides in a centrifugal ultrafiltration device prior to two-dimensional gel electrophoresis. Smejkal GB, Li C, Robinson MH, Lazarev AV, Lawrence NP, Chernokalskaya E. J Proteome Res. 2006 Apr;5(4):983-7. 2005 | Protein Concentration |
| Measurement of Pro-C-Type Natriuretic Peptide in Plasma Nielsen, S.J., Rehfeld, J.F., Pedersen, F., Kastrup, J., Videback, R., Goetze, J.P. Clinical Chemistry 51 (2005): 2173-2716 2004 | |
| Comparative Proteomic Analysis of Intra- and Interindividual Variation in Human Cerebrospinal Fluid Hu, Y., Malone, J.P., Fagan, A.M., Townsend, R.R., Holtzmann, D.M Molecular & Cellular Proteomics 4.12 (2005): 2000-2009 2004 | |
| Ultrafiltration for proteomic sample preparation E. Chernokalskaya, S. Gutierrez, A.M. Pit, J. T. Leonard Electrophoresis, 25: 2461-2468 2004 | Sample Prep |
FAQ
| Frage | Antwort | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Amicon Ultra units are offered in what volumes? | Amicon Ultra units are available in 0.5 mL, 4mL & 15ml volumes. | ||||||||||||
| What is the recommended rotor for Amicon Ultra units? | Any rotor capable of accommodating 22.7 cm in diameter and 124 mm length centrifuge tubes for the Amicon Ultra-4 and 35 mm in diameter and 121 mm in length for the Amicon Ultra-15 can be used for Amicon Ultra units. | ||||||||||||
| How should I select the appropriate Molecular Weight Cutoff (MWCO) for Amicon Ultra? | Select a Molecular Weight Cutoff that is 2x smaller that the Molecular Weight (MW) of the solute of interest. | ||||||||||||
| Which membranes are available in Amicon Ultra units? | Amicon Ultra units are only available with Ultracel regenerated cellulose material. This high recovery/low binding membrane is offered in 3,000, 10,000, 30,000, 50,000 & 100,000 MWCO. | ||||||||||||
| Can the Amicon Ultra be spun to dryness? | No, the Amicon Ultra has a built in deadstop. | ||||||||||||
| Can the Amicon Ultra be used with nucleic acids? | Yes, Amicon Ultra units have been evaluated for use with Genomic and PCR fragments. The 30 kDa MWCO provides the best balance between recovery and spin time. Protocols related to Genomic and PCR fragments can be found in the user guide. | ||||||||||||
| What is the maximum sample volume for Amicon Ultra? |
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| What is the maximum centrifugal force I can spin Amicon Ultra-4 & Amicon Ultra-15? |
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| What is the chemical compatibility of Amicon Ultra? | Amicon Ultra units are intended for use with biological fluids and aqueous solutions. Please refer to the user guide, catalogue, or contact Technical Support. | ||||||||||||
| Can Amicon Ultra units be sterilized with ethanol? | Amicon Ultra is compatible with 70% Ethanol, but we have not done any studies to test for sterilization. |
Verwandte Produkte & Anwendungen
Verwandte Produkte nach: Application Facete
| Protein Purification |
| Dialysis and Filtration |
Verwandte Produkte nach: Brand Facete
| Ultracel® |
| Amicon® |
Kategorien
| Life Science Research > Protein Sample Preparation > Protein Concentration & Dialysis > Sample Prep Centrifugal Filter Units |
- Die Ultracel Membran aus regenerierter Zellulose mit hoher Rückgewinnung in einer Vielzahl von Molekulargewichtsabscheidegrenzen
- Hohe Retentat-Rückgewinnung von >90 %
- Die vertikale Membran reduziert die Konzentrationspolarisation für extrem schnelle Zentrifugationszeiten (nur 5 Minuten).
- Die hitzeversiegelte Membran verringert die Freisetzung extrahierbarer Substanzen auf ein Mindestmaß.
- 100%ig integritätsgetestet für zuverlässige Leistung
- Optimale Überwachung der Proben durch durchsichtiges Gehäuse und deutliche Volumenskala
- Direkte Probenentnahme mittels Pipette erspart einen Verarbeitungsschritt für die Gewinnung des Konzentrats.
- Hohe Konzentrationsfaktoren von 80 - 100 x
Anwendungen
- Konzentrieren biologischer Proben, die Antigene, Antikörper, Enzyme, Nukleinsäuren oder Mikroorganismen enthalten.
- Aufreinigung makromolekularer Komponenten aus Zellkulturextrakten oder Zell-Lysaten
- Konzentrieren verdünnter oder vorgereinigter Proteine aus Chromatographie-Eluaten
- Entsalzen und Pufferaustausch
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Typische Zentrifugationszeiten zu Filtrationsvolumina
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Zentrifugationsbedingungen: 4000 xg Schwingkopfrotor bei 25 °C, Probenvol. 4 ml.
3K, 10K: Cytochrom c (0,25 mg/ml);
30K, 50K: BSA (1 mg/ml);
100K: IgG (1 mg/ml).
Typische Proteinrückgewinnung
| Membran NMGG, Da |
Protein in Lösung | Proteinretentat Volumen, ml |
Proteinretentat Rückgewinnung, % |
| 3.000 | Cytochrom c, 12.400 Da (0,25 mg/ml) |
0,19 | 98,7 |
| 10.000 | Cytochrom c, 12.400 Da (0,25 mg/ml) |
0,20 | 95 |
| 30.000 | BSA, 67.000 Da (1 mg/ml) |
0,20 | 95 |
| 50.000 | BSA, 67.000 Da (1 mg/ml) |
0,08 | 94 |
| 100.000 | IgG, 156.000 Da (1 mg/ml) |
0,34 | 91 |
| 4 ml Anfangsvolumen in einem Festwinkelrotor mit 7.500 xg bei 25 °C. Zentrifugationszeiten: 3K (30 min); 10 und 30K (10 min); 50K (5 min); 100K (15 min). | |||
| Volumina | |
| Maximales Anfangsvolumen der Probe, ml* | 4 mL |
| Typisches Endvolumen des Konzentrats, µl | 50 µL |
| Zentrifugalkraft | Schwingkopfrotor: 4.000 x g; Festwinkelrotor: 7.500 x g bei 3, 10, 30 und 50K; 5.000 x g bei 100K |
| Materialien | |
| Membran | Ultracel aus regenerierter Zellulose |
| Filtereinheit | Housing: Styrene/butadiene Röhrchen: Polypropylen Kappe mit Dichtungsmaterial: Polyethylen |
| Abmessungen | |
| Aktive Membranfläche, cm2 | 3 cm² |
| Filtrate tube or vial (centrifuge tube) | |
| Durchmesser | 17.3 mm |
| Länge (with cap and device) | 124 mm (4.88 in.) |
| Filter unit | |
| Diameter, mm | 17.2 |
| Length, mm | 73.4 |
| *Außer bei 23°-Festwinkelrotor: 3,5 ml | |