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SCM300 3dGRO® Organoid Dissociation Reagent

Chemically defined enzyme-free organoid dissociation solution.

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SCM300
100 ml  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberSCM300
      Description3dGRO® Organoid Dissociation Reagent
      Alternate Names
      • Organoid passaging reagent
      Background InformationOrganoids are in-vitro derived 3D cell aggregates derived from primary tissue or stem cells that are capable of self-renewal, self-organization and exhibit organ functionality. Organoids address the limitations of existing 2D model systems by providing:

      • Similar composition and architecture to primary tissue: Organoids harbor small population of self-renewing stem cells (such as intestinal crypt stem cells) that can differentiate into cells of all major cell lineages, with similar frequency as in physiological conditions.
      • Relevant models of in-vivo conditions: Organoids are more biologically relevant to any model system and are amenable to manipulate niche components and gene expression.
      • Stable system for extended cultivation: Organoids can be cryopreserved as biobanks and expanded indefinitely by leveraging self-renewal and the differentiation capability of the stem cell populations.

      The 3dGRO® Organoid Dissociation Reagent is a proprietary chemically defined enzyme-free dissociation solution used to passage multiple organoid cell types (etc. mouse intestinal organoids, human colon and lung organoids). Due to its chemically defined nature, the reagent produces consistent organoid passaging results. 3dGRO® Organoid Dissociation Reagent can be used in combination with the 3dGRO® R-Spondin-1 Conditioned Media Supplement (SCM104) to isolate and passage primary intestinal organoids from mice or in combination with the 3dGRO® Human iPSC Derived Colon Organoid Expansion Medium (SCM304) to expand human iPSC derived colon organoids (SCC300).
      References
      Product Information
      HS Code3824 99 89
      Applications
      ApplicationChemically defined enzyme-free organoid dissociation solution.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Biological Information
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceAppearance: Clear, Liquid
      Osmolality: 340-370 mOsm
      Sterility Tested: No Growth/Pass
      Endotoxin; <2 EU/ml
      pH: 7.2-7.4
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore the 3dGRO® Organoid Dissociation Reagent at room temperature. Aliquot into smaller working aliquots before use to avoid reagent contamination issues.
      Packaging Information
      Material Size100 ml
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      SCM300 04054839736209