Millipore Sigma Vibrant Logo
 

mouse+neural+stem+cell


121 Results 고급 검색  
검색결과 보기
제품 정보 (6)
문서 정보 (103)
웹 페이지 (12)

결과내 검색 검색을 미세조정하려면 아래의 필터를 이용하십시오

찾으시는 것을 발견할 수 없으십니까?
고객 서비스에 연락하십시오

 
문서 검색을 위한 도움이 필요하신가요?
  • CoA나 CoQ, MSDS 검색을 위해 문서 검색기를 이용해 보세요.
  • 사용자 가이드 또는 매뉴얼을 원하시면 고객 서비스팀에 연락주시기 바랍니다.

  • A neonatal mouse spinal cord injury model for assessing post-injury adaptive plasticity and human stem cell integration. 23990976

    Despite limited regeneration capacity, partial injuries to the adult mammalian spinal cord can elicit variable degrees of functional recovery, mediated at least in part by reorganization of neuronal circuitry. Underlying mechanisms are believed to include synaptic plasticity and collateral sprouting of spared axons. Because plasticity is higher in young animals, we developed a spinal cord compression (SCC) injury model in the neonatal mouse to gain insight into the potential for reorganization during early life. The model provides a platform for high-throughput assessment of functional synaptic connectivity that is also suitable for testing the functional integration of human stem and progenitor cell-derived neurons being considered for clinical cell replacement strategies. SCC was generated at T9-T11 and functional recovery was assessed using an integrated approach including video kinematics, histology, tract tracing, electrophysiology, and high-throughput optical recording of descending inputs to identified spinal neurons. Dramatic degeneration of axons and synaptic contacts was evident within 24 hours of SCC, and loss of neurons in the injured segment was evident for at least a month thereafter. Initial hindlimb paralysis was paralleled by a loss of descending inputs to lumbar motoneurons. Within 4 days of SCC and progressively thereafter, hindlimb motility began to be restored and descending inputs reappeared, but with examples of atypical synaptic connections indicating a reorganization of circuitry. One to two weeks after SCC, hindlimb motility approached sham control levels, and weight-bearing locomotion was virtually indistinguishable in SCC and sham control mice. Genetically labeled human fetal neural progenitor cells injected into the injured spinal cord survived for at least a month, integrated into the host tissue and began to differentiate morphologically. This integrative neonatal mouse model provides opportunities to explore early adaptive plasticity mechanisms underlying functional recovery as well as the capacity for human stem cell-derived neurons to integrate functionally into spinal circuits.
    문서 타입:
    Reference
    카탈로그 번호:
    Multiple
    제품명:
    Multiple