User Guide - Immobilon® FL Transfer Membrane
- 문서 타입:
- User Guide
- 카탈로그 번호:
- Multiple
- 제품명:
- Multiple
Immobilon FL
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Immobilon®-FL Transfer Membrane User Guide
- 문서 타입:
- User Guide
- 카탈로그 번호:
- Multiple
- 제품명:
- Multiple
-
Is Immobilon-FL better than Nitrocellulose membrane for Fluorescence detection?…
- Yes. Background fluorescence of Immobilon-FL is typically 2-5X lower than that of nitrocellulose, thus improving signal-to-noise ratio (sensitivity). Nitrocellulose membranes have other disadvantages. If allowed to dry out, they become brittle, tend to fracture and are difficult to handle. They are not recommended for stripping and re-probing. Nitrocellulose blots need to be scanned as soon as possible after detection, as diffusion of signal on a wet membrane may also occur.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper
-
Can Immobilon-FL be used with Millipore’s Rapid Immunodetection Method?…
- Yes. Immobilon-FL can be used with the Rapid Immunodetection Method. However slightly higher background may be observed.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper
-
Can Immobilon-FL be used with other detection chemistries (chromogenic, radioactive, and chemilumine…
- Immobilon-FL is optimized for fluorescence-based detection applications. It can be used with other western blotting detection methods, e.g., chromogenic, radioactive and chemiluminescent, using standard protocols.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper
-
Can Immobilon-FL be stripped and re-probed?…
- Yes, Immobilon-FL can be stripped and re-probed like any other PVDF membrane, using standard detergent or acidic methods (refer to Millipore’s Protein Blotting Handbook). Keep the blot wet. If dried, rewet with methanol and then rehydrate before stripping the blot. Once the membrane has dried the stripping is ineffective. Alternatively, the blot can be photo-bleached to remove the fluorescence.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper
-
Do I need to rinse the Immobilon-FL blotting membrane with water prior to blocking step, after wetti…
- It is not necessary to rinse the membrane with water. However, carry-over of methanol from the wetting step can reduce binding of blocking reagents to the membrane.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper
-
Do I need to dry Immobilon-FL blots before scanning?…
- Drying the blot may enhance signal strength. NOTE: Do not wrap the blot in plastic/saran wrap while scanning. If you do need to wrap the membrane, Mylar should be used.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper
-
If I need to write on an Immobilon-FL blot what marker should I use?…
- A pencil should be used for writing/marking on the blot. Ink from most pens and markers may fluoresce or wash off and redeposit elsewhere on the blot. You can also use marker pens supplied by scanner manufacturers.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper
-
What is the difference between Immobilon–FL and Immobilon-P?…
- Both membranes are made from PVDF. The difference in background fluorescence is due to proprietary modifications in the membrane manufacturing process.
- 문서 타입:
- FAQ
- 카탈로그 번호:
- C3117
- 제품명:
- Immobilon® Membranes, Sandwiches and Blotting Filter Paper