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Merck

SCR103

Collagenase type I

The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

동의어(들):

Collagenase Type I from Clostridium histolyticum, Tissue dissociation

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352204
NACRES:
NA.75
eCl@ss:
32160801
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제품 이름

Collagenase type I, The collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means.

form

powder

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)

shipped in

wet ice

Quality Level

Physical form

Collagenase type I is supplied as a dialyzed, lyophilized powder.

Analysis Note

Each lot of collagenase type I is assayed via a modification of Mandl (1964), with the extent of collagen breakdown determined using the colorimetric ninhydrin method of Moore and Stein (1948). Each lot is then tested for successful passage of human embryonic stem cells cultured in Millipore′s HEScGRO Medium for hES cells. Cells are characterized for correct marker expression and morphology after five passages.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

General description

Collagenase type I (from Clostridium histolyticum) is a crude collagenase preparation that can be used for the isolation of primary cells or for tissue dissociation by enzymatic means. The preparation also may contain caseinase, clostripain, and tryptic activities. This preparation can be used to dissociate cells in culture, and has been successfully used to passage human embryonic stem cells cultured with Millipore’s HEScGROTM Animal Component-Free Medium.

Preparation Note

Collagenase type I should be stored in its powdered form at 2-8oC until ready to use. Solutions of collagenase type I can be aliquotted and stored at -20oC, but should not be repeatedly thawed and re-frozen.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1 vial containing 250mg of lyophilized powder

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

저장 등급

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Yiya Yang et al.
International urology and nephrology, 53(6), 1247-1254 (2021-05-05)
High glucose (HG) induces the production of transforming growth factor (TGF)-β and reactive oxygen species, which further activates JAK/STAT signaling and promotes the synthesis of matrix proteins, contributes to the pathophysiological processes of diabetic nephropathy. This study aims to investigate
Lu-Lu Li et al.
Frontiers in cellular and infection microbiology, 10, 581535-581535 (2021-01-26)
Pseudomonas aeruginosa (PA) is one of the most common Gram-negative bacteria causing hospital-acquired pulmonary infection, with high drug resistance and mortality. Therefore, it is urgent to introduce new non-antibiotic treatment strategies. Mesenchymal stem cells (MSCs), as important members of the
Nigel S Michki et al.
Cell reports, 35(4), 109039-109039 (2021-04-29)
The Drosophila type II neuroblast lineages present an attractive model to investigate the neurogenesis and differentiation process as they adapt to a process similar to that in the human outer subventricular zone. We perform targeted single-cell mRNA sequencing in third
Jinfei Fu et al.
American journal of cancer research, 10(12), 4234-4250 (2021-01-09)
Cytotoxic CD8+ T cell exhaustion is one of the mechanisms underlying the tumor immune escape. The paradigm-shifting immune checkpoint therapy can mitigate CD8+ T lymphocyte exhaustion, reinvigorate the anticancer immunity, and achieve durable tumor regression for some patients. Emerging evidence
Qian He et al.
Frontiers in immunology, 11, 571248-571248 (2020-10-20)
As the entry sites of many pathogens such as human immunodeficiency virus (HIV), mucosal sites are defended by rapidly reacting resident memory T cells (TRM). TRMs represent a special subpopulation of memory T cells that persist long term in non-lymphoid

문서

조직 및 줄기세포 유래 3D 뇌, 장, 소화관, 폐 및 암 종양 오가노이드 모델을 생성하는 오가노이드 배양 제품.

Organoid culture products to generate tissue and stem cell derived 3D brain, intestinal, gut, lung and cancer tumor organoid models.

국제 무역 품목 번호

SKUGTIN
SCR10304053252397868

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