3dGRO^® R-Spondin-1 Conditioned Media Supplement

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

3D organoid culture systems are increasingly employed as powerful tools for the study of human diseases. R-Spondin proteins are a family of cysteine-rich, thrombospondin type I repeat containing proteins that activate both the WNT/β-catenin and WNT/PCP pathways. R-spondin-1 (RSPO1) is one of the most extensively used niche factors for culturing 3D organoids and has been used to establish organoids from the stomach, small intestine, colon, pancreas and liver from both mouse and human sources^1,2,3 along with intestinal organoids from other large and small animals⁴. However, due to the high concentration requirement in organoid media the use of the recombinant R-spondin-1 becomes prohibitively expensive. The 3dGRO^® R-Spondin-1 Conditioned Media Supplement is a potent and inexpensive alternative to purified recombinant RSPO1 protein for organoid cultures and is optimized for the highest level of RSPO-1 expression to ensure consistency and performance.



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The 3dGRO^® R-Spondin-1 Conditioned Media Supplement is generated from a 293T cell transfectant stably secreting mouse R-spondin-1 tagged with an influenza hemagglutinin (HA) epitope at the N-terminus and a murine IgG2a Fc fragment at the C-terminus. The conditioned medium does not contain serum.

RSPO-1 Conditioned Medium should be stored at -20°C. When ready to use, thaw the supplement overnight at 2-8°C in the dark. Thawed RSPO-1 Conditioned Medium is good for two weeks when stored at 2-8°C.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany