AT-3 Mouse Mammary Carcinoma Cell Line

• Each vial contains ≥ 1X10⁶ viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination

AT-3 mouse mammary tumor cell line may be used to develop mouse models for mammary cancer studies.

Research Category
Cancer

Oncology

This product is sold solely for research use per the terms of the “Restricted Use Agreement” which govern its use as detailed in the product Data Sheet or Certificate of Analysis. For information regarding any other use, please contact licensing@emdmillipore.com.

Cancer Cells

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

The AT-3 cell line was isolated from an autologous mammary tumor from cells originating in a transgenic MTAG mouse model . Mammary tumors arising in MTAG mice closely mimic changes observed in human breast carcinomas over the course of disease progression . AT-3 cells are a useful mouse model for studies of tumor physiology that are representative of typical disease states in humans.

Source:
The AT-3 cell line was derived from primary mammary tumor cells of a transgenic MTAG mouse. The MTAG mouse model expresses the polyoma virus middle T antigen under control of the MMTV-LTR promoter .

Note: AT-3 cells demonstrate diverse morphologies in culture.

Tumor-induced immunosuppression is a common phenomenon in cancer and an important consideration for cancer immunotherapy. However, most studies on tumor-induced immunosuppression rely on tumor implant models, in which the disease progresses rapidly and usually causes non-physiological host responses that do not recapitulate normal tumor development and progression . Models that generate autochthonous tumors are therefore valuable for providing more physiologically-relevant systems for investigating tumor-host interactions and longer-term immunosuppressive effects.

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.