CC077
Human Collagen Type V
from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation
동의어(들):
Collagen Type V
조직 및 계약 가격을 보려면 로그인를 클릭합니다.
크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.75
Biological source:
human
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Concentration:
1 mg/mL
Assay:
95% (SDS-PAGE)
제품 이름
Human Collagen Type V, 1 mg/mL, solution, BioReagent, suitable for cell culture
biological source
human
assay
95% (SDS-PAGE)
form
solution
manufacturer/tradename
Chemicon®
concentration
1 mg/mL
technique(s)
cell culture | mammalian: suitable
impurities
<0.5% non-collagen proteins
<1% collagen type III
<2% collagen type I
<2% collagen type IV
input
sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
human ... COL5A1(1289), COL5A2(1290)
Application
Human Collagen Type V has been used to study the interaction of high endothelial venule protein (hevin) with human collagen V by fluorescence polarization (FP) assay and surface plasmon resonance(SPR). It has also been used to incubate the 96 well plate to determine the protein levels in media and cell lysates of fibroblasts.
Biochem/physiol Actions
Collagen type V acts as a regulator of collagen fibrillogenesis in the cornea and skin dermis. It interacts with matrix collagens and structural proteins thereby contributing to the structural integrity of tissues. Lower levels of type V collagen lead to loss of corneal transparency and Ehler Danlos syndrome.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
General description
Collagen type V is fibril-forming collagen that is located in the lung, bone, and fetal membranes along with type I collagen. It is minor collagen present in the extracellular matrix.
Other Notes
Molecular composition: α1(V)]2 α2(V), native triple helix. Purity and retention of the native helical structure were monitored by SDS-PAGE, ORD measurement, and by reaction with anti-collagen type-specific monoclonal antibodies. Product Source: Human placenta, negative for HBsAg and HIV antibodies.
Physical form
Liquid, in 0.1M acetic acid, pH 3.0. No preservatives added.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months. Do not thaw and refreeze.
Purified by serial salt precipitations of a pepsin extraction of human fetal membranes and chromatography on DEAE-cellulose.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
K Gelse et al.
Advanced drug delivery reviews, 55(12), 1531-1546 (2003-11-19)
The extracellular matrix represents a complex alloy of variable members of diverse protein families defining structural integrity and various physiological functions. The most abundant family is the collagens with more than 20 different collagen types identified so far. Collagens are
The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
Klasson, S C, et al.
Collagen and Related Research, 6, 397-408 (1986)
Type V collagen in health, disease, and fibrosis
The Anatomical Record, 299(5), 613-629 (2016)
Neena Philips et al.
Connective tissue research, 53(5), 373-378 (2012-02-14)
Skin aging is associated with the loss of the structural collagens and the elastin fiber components that form the extracellular matrix (ECM). It is associated with reduced transforming growth factor-β (TGF-β), angiogenesis and increased oxidative stress. Copper has been incorporated
Shanghua Fan et al.
Structure (London, England : 1993), 29(7), 664-678 (2021-02-04)
Hevin is secreted by astrocytes and its synaptogenic effects are antagonized by the related protein, SPARC. Hevin stabilizes neurexin-neuroligin transsynaptic bridges in vivo. A third protein, membrane-tethered MDGA, blocks these bridges. Here, we reveal the molecular underpinnings of a regulatory network
프로토콜
This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| CC077 | 04053252327070 |
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