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Merck

ABE1047

Anti-NRF2a/GABPA Antibody

serum, from rabbit

동의어(들):

GA-binding protein alpha chain, GABP subunit alpha, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60, NRF2a/GABPA

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
ChIP
electrophoretic mobility shift assay
western blot
Species reactivity:
human
Citations:
5
Technique(s):
ChIP: suitable
electrophoretic mobility shift assay: suitable
western blot: suitable
Uniprot accession no.:
Q06546

주요 문서

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제품 이름

Anti-NRF2a/GABPA Antibody, serum, from rabbit

Quality Level

100

biological source

rabbit

conjugate

unconjugated

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human

technique(s)

ChIP: suitable
electrophoretic mobility shift assay: suitable
western blot: suitable

NCBI accession no.

NP_001184226

UniProt accession no.

Q06546

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... GABPA(2551)

Analysis Note

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected NRF2a/GABPA in 10 µg of HeLa cell lysate.

Application

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected GABPA (NRF-2alpha) occupancy at the TFB1M and TFB2M promoters by ChIP using HeLa chromatin preparations (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Western Blotting Analysis: A representative lot detected GABPA (NRF-2alpha) in U2OS cell lysates (Gleyzer, N., and Scarpulla, R.C. (2011). J. Biol. Chem. 286(46):39715-39725; Gleyzer, N., and Scarpulla, R.C. (2013). J. Biol. Chem. 288(12):8004-8015).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using recombinant GABPA or heparin agarose-purified HeLa nuclear extract and radiolabeled synthetic oligonucleotides corresponding to cytochrome oxidase subunit IV, hTFB1M, or hTFB2M promoter sequence with GABPA recognition sites (Gleyzer, N., et al. (2005). Mol Cell Biol. 2005 Feb;25(4):1354-66).
Electrophoretic Mobility Shift Assay (EMSA) Analysis: A representative lot caused a supershift of GABPA (NRF-2alpha)-DNA complex in EMSA using in vitro translated GABPA and radiolabeled cytochrome oxidase subunit IV promoter fragment containing tandem GABPA recognition sites (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
Immunoprecipitation Analysis: A representative lot immunoprecipitated GABPA (NRF-2alpha)-PRC complex from human embryonic kidney 293FT cell lysate (Vercauteren, K., et al. (2008). J Biol Chem. 283(18):12102-12111).
This Anti-NRF2a/GABPA Antibody is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Electrophoretic Mobility Shift Assay for the detection of NRF2a/GABPA.

General description

GA-binding protein alpha chain (UniProt Q06546; also known as GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor 2 subunit alpha, Transcription factor E4TF1-60) is encoded by the GABPA (Also known as E4TF1A) gene (Gene ID 2551) in human. Initially identified through their interactions with cytochrome c and cytochrome oxidase promoters, GABPA (NRF-2) and the related nuclear respiratory factor NRF-1 target many nuclear genes essential for mitochondrial respiratory function, including the mitochondrial transcription specificity factors TFB1M and TFB2M. GABPA plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPA is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
~55 kDa observed

Immunogen

Recombinant protein corresponding to human NRF2a/GABPA.

Other Notes

Concentration: Please refer to lot specific datasheet.

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저장 등급

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

시험 성적서(COA)

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문서 라이브러리 방문

Tongwu Zhang et al.
Cancer research, 77(7), 1649-1661 (2017-01-22)
SDHD encodes subunit D of the succinate dehydrogenase complex, an integral membrane protein. Across cancer types, recurrent SDHD promoter mutations were reported to occur exclusively in melanomas, at a frequency of 4% to 5%. These mutations are predicted to disrupt
Andrew M McKinney et al.
Cell reports, 40(12), 111344-111344 (2022-09-22)
Telomerase activation counteracts senescence and telomere erosion caused by uncontrolled proliferation. Epidermal growth factor receptor (EGFR) amplification drives proliferation while telomerase reverse transcriptase promoter (TERTp) mutations underlie telomerase reactivation through recruitment of GA-binding protein (GABP). EGFR amplification and TERTp mutations
Johan O Paulsson et al.
Endocrine-related cancer, 27(5), 295-308 (2020-03-13)
Mutations in the miRNA enzyme gene DICER1 have been reported in several endocrine malignancies and is associated with the rare tumour-predisposing DICER1 syndrome. DICER1 mutations have been reported in subsets of follicular thyroid carcinoma (FTC), but the role of DICER1

관련 콘텐츠

Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System

A major focus of breast cancer research is to understand the mechanisms responsible for disease progression and drug resistance. Toward that end, it has been found that approximately two thirds of all human breast carcinomas overexpress the Estrogen Receptor α (ERα) protein and it remains the primary pharmacological target for endocrine therapy1,2. The normal cellular function of ERα is as a transcription factor that mediates a wide variety of physiological processes, many of which are dependent upon phosphorylation of the receptor at specific amino acid residues3,4. Indeed, ERα is known to be phosphorylated at a multitude of different sites, yet how these all correlate to disease remains unclear5. Here, we interrogated multiple sites of ERα for phosphorylation status by screening an extensive panel of different breast cancer patient samples and other non-breast cancer tissue microarray (TMA) slide samples to determine their relevance to disease.

White Paper: Further considerations of antibody validation and usage.

국제 무역 품목 번호

SKUGTIN
ABE104704055977181562

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