AB1541
Anti-Tryptophan Hydroxylase Antibody
CHEMICON®, sheep polyclonal
동의어(들):
Tryptophan 5-monooxygenase 1, indoleacetic acid-5-hydroxylase, tryptophan 5-hydroxylase, tryptophan 5-monooxygenase, tryptophan hydroxylase (tryptophan 5-monooxygenase), tryptophan hydroxylase 1
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크기 선택
제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
human, rat
Application:
immunohistochemistry
western blot
western blot
Technique(s):
immunohistochemistry: suitable
western blot: suitable
western blot: suitable
Citations:
81
Uniprot accession no.:
제품 이름
Anti-Tryptophan Hydroxylase Antibody, Chemicon®, from sheep
biological source
sheep
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, rat
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TPH1(7166)
Analysis Note
Control
Brain tissue
PC12 lysates
Brain tissue
PC12 lysates
Routinely evaluated by Western Blot on PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Tryptophan Hydroxylase on 10 μg of PC12 lysates.
Application
Detect Tryptophan Hydroxylase using this Anti-Tryptophan Hydroxylase Antibody validated for use in IH & WB.
Immunohistochemistry(peroxidase):
Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining.
Optimal working dilutions must be determined by the end user.
Performed on formaldehyde fixed tissue of a previous lot. Useful for cell body but not fiber staining.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species.
Recognizes Tryptophan Hydroxylase. Reacts with human and rat brain tissues by immunoblotting and immunohistochemistry. Based on the relatively high degree of homology of tryptophan hydroxylase, the antibody should cross-react with other mammalian species. Recently studies have indicated that AB1541 can cross react with Tyrosine hydroxylase on western blots, weakly. Cross reactivity in the native state is untested.
General description
55 kDa
Tryptophan hydroxylase (TPH) is an enzyme involved in the synthesis of the neurotransmitter serotonin. It is responsible for addition of the -OH group (hydroxylation) to the 5 position to form the amino acid 5-hydroxytryptophan (5-HTP), which is the initial and rate-limiting step in the synthesis of the hormone serotonin. It is also the first enzyme in the synthesis of melatonin.
Immunogen
Recombinant rabbit tryptophan hydroxylase, isolated as inclusion bodies from E. coli and purified by preparative SDS-PAGE.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB1556
Physical form
Purified sheep polyclonal liquid in 150 mM NaCl, 10 mM HEPES (pH 7.5), 100 μg/mL BSA and 50% glycerol.
Preparation Note
Stable for 6 months at -20ºC to -80ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
10 - Combustible liquids
wgk
WGK 2
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Carla Frare et al.
Frontiers in physiology, 9, 1747-1747 (2019-01-09)
Thermoregulation is necessary to maintain energy homeostasis. The novel discovery of brown adipose tissue (BAT) in humans has increased research interests in better understanding BAT thermogenesis to restore energy balance in metabolic disorders. The hibernating Arctic ground squirrel (AGS) offers
S J Bang et al.
Neuroscience, 179, 1-8 (2011-02-01)
Adolescence is a critical vulnerable period during which exposure to nicotine greatly enhances the possibility to develop drug addiction. Growing evidence suggests that serotonergic (5-HT) neurotransmission may contribute to the initiation and maintenance of addictive behavior. As the dorsal raphe
Olivier Berton et al.
Neuron, 55(2), 289-300 (2007-07-21)
We analyzed the influence of the transcription factor DeltaFosB on learned helplessness, an animal model of affective disorder wherein a subset of mice exposed to inescapable stress (IS) develop a deficit in escape behavior. Repeated IS induces DeltaFosB in the
Extrasynaptic glycine receptors of rodent dorsal raphe serotonergic neurons: a sensitive target for ethanol.
Maguire, EP; Mitchell, EA; Greig, SJ; Corteen, N; Balfour, DJ; Swinny, JD; Lambert, JJ; Belelli, D
Neuropsychopharmacology null
Aki Takahashi et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 35(16), 6452-6463 (2015-04-24)
Although the dorsal raphe nucleus (DRN) has long been linked to neural control of aggression, little is known about the regulatory influences of the DRN when an animal engages in either adaptive species-typical aggressive behavior or escalated aggression. Therefore it
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| AB1541 | 04053252626227 |
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