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Merck

1.04030

VRB (Violet Red Bile Lactose)-MUG agar

FluoroCult®, FDA-BAM, suitable for microbiology, for Escherichia coli, for coliforms

동의어(들):

Coliform selective agar, MUG Violet Red Bile Agar, VRB-MUG agar, Violet Red Bile Agar with MUG, Violet Red Bile Lactose-MUG agar

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

NACRES:
NA.74
UNSPSC Code:
41106200

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제품 이름

VRB (Violet Red Bile Lactose)-MUG agar, FluoroCult®, FDA-BAM, suitable for microbiology, for Escherichia coli, for coliforms

agency

FDA-BAM

form

medium granules (dehydrated (DCM))

packaging

pkg of 500 g

manufacturer/tradename

FluoroCult®

storage condition

(Keep tightly closed and dry.)

technique(s)

microbiological culture: suitable

color

pink

pH

7.3-7.5 (25 °C, 39.6 g/L in H2O)

solubility

39.6 g/L (after autoclaving)

bulk density

620 kg/m3

application(s)

food and beverages
microbiology

storage temp.

15-25°C

suitability

Escherichia coli
coliforms

Quality Level

200

관련 카테고리

General description

Fluorocult® VRB-MUG Agar is a selective medium for the detection and enumeration of coliform bacteria, in particular E. coli. Meat peptone provides amino acids and other complex nitrogenous substances, and yeast extract supplies Vitamin B complexes. Lactose is a fermentable carbohydrate. Crystal violet and bile salts inhibit the growth of accompanying Gram-positive bacterial flora. Neutral red acts as a pH indicator. Lactose-positive colonies show a color change to red due to acid production. Lactose-negative Enterobacteriaceae are colorless. Lactose-positive colonies are red and often surrounded by a turbid zone due to the precipitation of bile acids. E. coli colonies appear fluorescent under UV light. β-D-glucuronidase, which is produced by E. coli, cleaves 4-methylumbelliferyl-β-D-glucuronide (MUG) to 4-methylumbelliferone and glucuronide. The fluorogenic 4-methylumbelliferone can be detected under a long-wavelength UV lamp.

Legal Information

FLUOROCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

Appearance (clearness): clear
Appearance (color): dark red
pH-value (25 °C): 7.2 - 7.6
Solidification behaviour (2 hrs., 45 °C): liquid
Growth promotion test in accordance with the current version of DIN EN ISO 11133.

Growth(Escherichia coli ATCC 8739 (WDCM 00012)): good to very good
Growth Escherichia coliATCC 25922 (WDCM 00013): good to very good
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): no limit
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009)): total inhibition
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087)): total inhibition
Colonies (Escherichia coli ATCC 8739 (WDCM 00012)): red with precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013)): red with precipitation halo
Colonies (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): colourless to beige
Fluorescence (Escherichia coli ATCC 8739 (WDCM 00012)): + (poor)
Fluorescence (Escherichia coli ATCC 25922 (WDCM 00013)): +
Fluorescence (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): + (greenish)
Incubation: 18 - 24 hours at 35 °C aerobic.

Application

Fluorocult® VRB Agar is suitable for the detection and enumeration of coliform bacteria, particularly E. coli, in food and dairy products.

Features and Benefits

  • Fluorocult® fluorogenic culture media offer Rapid and Reliable culture testing
  • Powerful alternatives to conventional microbiological methods for the rapid and sensitive detection of bacteria
  • Offers superior granulated culture media
  • Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
  • Excellent wettability, solubility, and free-flowing properties
  • Convenient, with minimal component separation and clumping, even under warm or humid conditions
  • High batch-to-batch reproducibility
  • Prolonged shelf life of up to five years
  • High number of test strains exceeding all regulatory demands

저장 등급

11 - Combustible Solids

wgk

WGK 3

시험 성적서(COA)

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문서 라이브러리 방문

문서

Microbial Detection Using Chromogenic and Fluorogenic Culture Media

Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms

국제 무역 품목 번호

SKUGTIN
1.04030.050004022536046556

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