69671
Thrombin, Restriction Grade
동의어(들):
Thrombin, Restriction Grade
biological source
human plasma
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
technique(s)
protein extraction: suitable
suitability
suitable for additive or modifier in the separation of proteins or peptides
application(s)
life science and biopharma
shipped in
wet ice
storage temp.
−20°C
Quality Level
Disclaimer
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
General description
Restriction Grade Thrombin is a highly purified preparation qualified to specifically cleave target proteins produced with appropriate vectors. This preparation is functionally tested for activity with fusion proteins and is free of detectable contaminating proteases. Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. Thrombin is supplied as a solution in 50% glycerol and includes 10X Thrombin Cleavage Buffer and a Cleavage Control Protein.
Other Notes
•50 UThrombin
•1 ml10X Thrombin Cleavage Buffer
•2 ml1X Thrombin Dilution/Storage Buffer
•10 µgCleavage Control Protein
•1 ml10X Thrombin Cleavage Buffer
•2 ml1X Thrombin Dilution/Storage Buffer
•10 µgCleavage Control Protein
One unit is defined as the amount of enzyme needed to cleave 1 mg of fusion protein in 16 hours at 20°C in a 200 µl reaction containing 20 mM Tris-HCl pH 8.4, 150 mM NaCl, 2.5 mM CaCl₂, 50 µg fusion protein.
Preparation Note
Prepared from human plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
저장 등급
10 - Combustible liquids
wgk
WGK 1
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Jooyoung Park et al.
eLife, 8 (2019-12-20)
The computational design of a symmetric protein homo-oligomer that binds a symmetry-matched small molecule larger than a metal ion has not yet been achieved. We used de novo protein design to create a homo-trimeric protein that binds the C3 symmetric
Christian M Smolko et al.
Scientific reports, 9(1), 19409-19409 (2019-12-21)
Protein kinases are enzymes whose abundance, protein-protein interactions, and posttranslational modifications together determine net signaling activity in cells. Large-scale data on cellular kinase activity are limited, because existing assays are cumbersome, poorly sensitive, low throughput, and restricted to measuring one
문서
Find highly-specific cleavage enzymes for isolating proteins during fusion protein purification including enterokinase, factor Xa, HRV 3C protease, and thrombin.
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 69671-3CN | 04055977274189 |
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