1.06177
TBE buffer
10 x pH 8.3 tris-borate-EDTA buffer
동의어(들):
10X TBE running buffer, Tris-Borate EDTA buffer
제품 이름
TBE buffer, 10 x pH 8.3 tris-borate-EDTA buffer
form
liquid
pH
8.2-8.4 (20 °C in H2O)
density
1.06 g/cm3 at 20 °C
storage temp.
15-25°C
Analysis Note
Appearance (colour): almost colourless
Appearance (description): almost clear
pH-value: 8.2 - 8.4
Appearance (description): almost clear
pH-value: 8.2 - 8.4
General description
Tris, borate, and ethylenediaminetetraacetic (TBE) buffer is routinely used to conduct DNA and RNA agarose gel electrophoresis. It comprises a weak acid in neutral and anionic forms (e.g., COOH and COO- species) and a weak base, Tris, which is either in neutral or cationic forms (Tris-NH2 and Tris-NH3+). These ions buffer the pH, maintain a low conductivity medium, and transfer the electrical current. Ethylenediaminetetraacetic (EDTA) is used to chelate Mg2+ ions and inactivate the potential DNA nucleases. TBE is also used to perform a good resolution of DNA fragments that slightly enhances the separation of smaller fragments. It also protects nucleic acids from enzymatic degradation.
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Repr. 1B - Skin Sens. 1
저장 등급
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Optical properties of buffers and cell culture media for optofluidic and sensing applications
Hoang VT, et al.
Applied Sciences, 9(6), 1145-1145 (2019)
Brian A Sanderson et al.
Analytical biochemistry, 454, 44-52 (2014-03-19)
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric
국제 무역 품목 번호
| SKU | GTIN |
|---|---|
| 1.06177.2500 | 04022536706344 |
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