Millipore Sigma Vibrant Logo

HTS061RTA Ready-to-Assay™ CysLT1 Leukotriene Receptor Frozen Cells

Human CysLT1 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: Chem-1
HTS061RTA
1 kit  
가격 검색...
가격을 검색할 수 없습니다
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
가격 문의 추가 정보
()이 할인됨
 
—
가격 문의
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      특별 제공

       

      고객 서비스로 문의

      개요

      Replacement Information

      특별 제공

      This product requires a User Agreement (Label License). Please see product datasheet for further information

      주요 사양표

      SpeciesHost CellsProtein Target
      Human Chem-1CysLT1
      Description
      Catalogue NumberHTS061RTA
      Trade Name
      • Ready-to-Assay
      DescriptionReady-to-Assay™ CysLT1 Leukotriene Receptor Frozen Cells
      OverviewTRANSFECTION: Full-length human CYSLTR1 cDNA
      Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

      The cysteinyl leukotrienes, leukotriene C4, leukotriene D4 and leukotriene E4, are arachidonic acid derivatives modified by glutathione, Cys-Gly or Cys. Activated mast cells release cysteinyl leukotrienes, which cause smooth muscle contraction, airway constriction, and vascular permeability. The biological effects of the cysteinyl leukotrienes are mediated by two GPCRs, CysLT1 and CysLT2. A CysLT1 selective antagonist, montelukast, is used clinically in the treatment of asthma (Brink et al., 2003; Evans, 2002). Chemicon's cloned human CysLT1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CysLT1 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CysLT1 and its ligands.
      References
      Product Information
      Components
      • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
      • Fifty (50) mL of Media Component.
      Quality LevelMQ300
      Applications
      ApplicationHuman CysLT1 GPCR frozen cells for Calcium Flux FLIPR Assays.
      Key Applications
      • Calcium Flux Assays
      Biological Information
      GPCR ClassA
      Host CellsChem-1
      Protein TargetCysLT1
      Target Sub-FamilyLeukotriene
      Cell Line Type
      • GPCR Cell Lines
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsPlace cells in liquid nitrogen immediately upon receipt.
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      HTS061RTA 04053252629938

      Documentation

      관련 제품 및 어플리케이션

      제품 패밀리

      ChemiSCREEN™ GPCR Stable Cell Lines, Membranes Preparation and Ready-to-Assay™ Frozen Cells

      Millipore offers a large selection of robust and reliable G-protein coupled receptor products, including Stable Cell Lines, Membrane Preparations, and Frozen Cells. See below for GPCR research tools.
      더 보기 >>

      관련 제품: Brand Facete

      Ready-to-Assay™

      카테고리

      Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
      Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells