수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
			96-Well Plate

수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

71092 MilliporePopCulture^® Reagent

PopCulture^® Reagent MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Brochures
User Protocol
Citations

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개요

Replacement Information

가격 및 재고여부

카탈로그 번호	재고 정보	패킹	포장 단위	가격(VAT 별도)	수량
71092-3CN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Plastic ampoule	15 ml	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가
71092-4CN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Plastic ampoule	75 ml	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가
71092-5CN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Plastic ampoule	250 ml	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가

Description
Overview	PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium. Using this method, cell culture, protein extraction and purification performed in the original culture tube or multiwell plate. PopCulture perforates the E. coli cell wall without denaturing soluble protein and protects protein from the pH extremes produced in high density culture media. Recombinant proteins can be assayed directly or purified by adding an affinity matrix, washing the matrix:target protein complex to remove culture medium and cellular contaminants and eluting the purified protein from the matrix. Purification of fusion proteins from total culture extracts has been demonstrated using both IMAC and GST affinity chromatography methods (1). To further enhance the PopCulture purification procedure, lysozyme and/or Benzonase^® Nuclease may be added. Lysozyme cleaves a peptidoglycan bond in the E. coli cell wall, enhancing cell lysis and increasing the yield of protein (1, 2). Proteins may be expressed in a host encoding T7 lysozyme (pLysS host) or exogenous lysozyme may be added after the PopCulture Reagent. Benzonase Nuclease may also be added to degrade endogenous nucleic acids that may interfere with purification due to high viscosity. The PopCulture protein purification procedure is ideally suited to high throughput (HT) robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience. The magnetic agarose capture resins (e.g. GST•Bind™ Magnetic Agarose Beads, His•Bind^® Magnetic Agarose Beads) are optimal for HT applications since the entire procedure can be performed in a single tube without the need for columns or centrifugation. *patent pending
Catalogue Number	71092
Brand Family	Novagen®

Description

Overview

PopCulture Reagent* is a buffered mixture of concentrated detergents formulated to extract proteins from E. coli cells directly in their culture medium. Using this method, cell culture, protein extraction and purification performed in the original culture tube or multiwell plate. PopCulture perforates the E. coli cell wall without denaturing soluble protein and protects protein from the pH extremes produced in high density culture media. Recombinant proteins can be assayed directly or purified by adding an affinity matrix, washing the matrix:target protein complex to remove culture medium and cellular contaminants and eluting the purified protein from the matrix. Purification of fusion proteins from total culture extracts has been demonstrated using both IMAC and GST affinity chromatography methods (1).

To further enhance the PopCulture purification procedure, lysozyme and/or Benzonase^® Nuclease may be added. Lysozyme cleaves a peptidoglycan bond in the E. coli cell wall, enhancing cell lysis and increasing the yield of protein (1, 2). Proteins may be expressed in a host encoding T7 lysozyme (pLysS host) or exogenous lysozyme may be added after the PopCulture Reagent. Benzonase Nuclease may also be added to degrade endogenous nucleic acids that may interfere with purification due to high viscosity.

The PopCulture protein purification procedure is ideally suited to high throughput (HT) robotic processing of samples for proteomics research or any application that would benefit from the increased speed and convenience. The magnetic agarose capture resins (e.g. GST•Bind™ Magnetic Agarose Beads, His•Bind^® Magnetic Agarose Beads) are optimal for HT applications since the entire procedure can be performed in a single tube without the need for columns or centrifugation.

*patent pending

Catalogue Number

71092

Brand Family

Novagen®

References
References	1. Grabski, A., Drott, D., Handley, M., Mehler, M. and Novy, R. (2001). inNovations 13, 1–4. 2. Inouye, M., Arnheim, N. and Sternglanz, T. (1973). J. Biol. Chem. 248, 7247.

Product Information
Declaration	This product is covered by EP Patent 1,432,822 owned by EMD Chemicals Inc. or its Affiliates.
Quality Level	MQ100

Applications

Biological Information

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Shipped at Ambient Temperature or with Blue Ice
Toxicity	Irritant
Storage	+15°C to +30°C
Do not freeze	Ok to freeze

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
카탈로그 번호	GTIN
71092-3CN	04055977271799
71092-4CN	04055977271812
71092-5CN	04055977271829

Documentation

PopCulture^® Reagent MSDS

타이틀
물질안전보건자료(MSDS)

PopCulture^® Reagent Certificates of Analysis

Title	Lot Number
71092	로트(lot) 번호를 입력하십시오.

References

참고문헌 보기
1. Grabski, A., Drott, D., Handley, M., Mehler, M. and Novy, R. (2001). inNovations 13, 1–4. 2. Inouye, M., Arnheim, N. and Sternglanz, T. (1973). J. Biol. Chem. 248, 7247.

Brochure

Title
Bulk Product Guide
Overnight Express Autoinduction Systems
Protein Purification and Detection Tools

Citations

타이틀

Andrew T. Anfora and Rodney A. Welch. (2006) DsdX is the second D-serine transporter in uropathogenic Escherichia coli clinical isolate CFT073. Journal of Bacteriology 188, 6622-6628.

Ilya G. Serebriiskii, et al. (2005) A combined yeast/bacterial two-hybrid system: development and evaluation. Molecular and Cellular Proteomics 4, 819-826.

Henry Nguyen, et al. (2004) An automated small-scale protein expression and purification screening provides beneficial information for protein production. Journal of Structural and Functional Genomics 5, 23-27.

User Protocols

Title
TB323 PopCulture^® Reagent

카테고리

Life Science Research > Protein Sample Preparation > Protein Extraction > Cell Lysis > Yeast and Plant

해당 제품은 고객님의 카트에 추가되었습니다.

71092 MilliporePopCulture^® Reagent

Recommended Products

개요

가격 및 재고여부

Documentation

PopCulture^® Reagent MSDS

PopCulture^® Reagent Certificates of Analysis

References

Brochure

Citations

User Protocols

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가격 및 재고여부

PopCulture® Reagent MSDS

PopCulture® Reagent Certificates of Analysis

References

Brochure

Citations

User Protocols

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PopCulture^® Reagent MSDS

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