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506119 PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

This PhosphoDetect™ Anti-p38 MAP Kinase Rabbit pAb is validated for use in WB, ICC for the detection of p38 MAP Kinase .

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506119
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개요

주요 사양표

Species ReactivityHostAntibody Type
Ca, H, M, RRbPolyclonal Antibody

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506119-10TCN
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      		 Plastic ampoule 10 t
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      Description
      OverviewRecognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
      Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number506119
      Brand Family Calbiochem®
      Application Data
      Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
      References
      ReferencesMarinissen, M.J., et al. 2001. Genes Dev. 15, 535.
      Nakagami, H., et al. 2001. Diabetes 50, 1472.
      Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
      Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
      Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
      Han, J., et al. 1994. Science 265, 808.
      Lee, J.C., et al. 1994. Nature 372, 739.
      Rouse, J., et al. 1994. Cell 78, 1027.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Positive controlUV irradiated HEK293 cells or sorbitol-treated PC12 cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesImmunoblotting (1:1000)
      Immunocytochemistry (see comments)
      Application CommentsDoes not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Canine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      카탈로그 번호 GTIN
      506119-10TCN 04055977199451