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GF149 PDGF-BB, recombinant human

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

PDGF-BB, recombinant human MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Culture UniProt Number: P01127, P31240 (mouse), Q05028 (rat)
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GF149
10 µg  
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      개요

      Replacement Information

      주요 사양표

      Key ApplicationsSpeciesSourcePurity
      CULTHuman E. coliGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
      Description
      Catalogue NumberGF149
      ReplacesGF018
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionPDGF-BB, recombinant human
      Alternate Names
      • Platelet Derived Growth Factor-BB
      Background InformationPlatelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).
      References
      Product Information
      Formulation
      PresentationSterile filtered solution lyophilized from 10 mM HOAc.
      Quality LevelMQ100
      Applications
      ApplicationThe Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.
      Key Applications
      • Cell Culture
      Application NotesFor most in vitro applications, PDGF-BB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): fibroblasts, connective tissue.
      Biological Information
      PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/μg) of PDGF-BB.
      SourceE. coli
      Specific ActivityHuman PDGF-BB is fully biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells is between 6-12 ng/mL.
      Gene Symbol
      • PDGFB
      • PDGF2
      • SIS
      UniProt Number
      Stem Cell Type
      • Neural Stem Cells
      • Mesenchymal Stem Cells
      • Hematopoietic Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-BB should be stored in undiluted aliquots at -20°C for up to six months.
      Packaging Information
      Material Size10 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      GF149 04053252275746

      Documentation

      PDGF-BB, recombinant human MSDS

      타이틀

      물질안전보건자료(MSDS) 

      PDGF-BB, recombinant human Certificates of Analysis

      TitleLot Number
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 1997734 1997734
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 2120320 2120320
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 2250502 2250502
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 2975576 2975576
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3307477 3307477
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3448673 3448673
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3683899 3683899
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - 3884316 3884316
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - DAM1593819 DAM1593819
      PDGF-BB, HUMAN RECOMBINANT PROTEIN - DAM1770456 DAM1770456
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      Data Sheet

      Title
      PDGF-BB, HUMAN RECOMBINANT PROTEIN
      Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

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      Cytokines and Growth Factors

      EMD Millipore offers a comprehensive range of highly purified bioactive cytokines and growth factors for use in human, mouse, and rat cell culture, stem cell differentiation and ELISA studies.
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      카테고리

      Life Science Research > Proteins and Enzymes > Cytokines & Growth Factors
      Life Science Research > Cell Culture and Systems > Cell Culture Media and Supplements > Cell Culture Supplements > Cytokines & Growth Factors