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EZMADP-60BK Mouse Adiponectin ELISA- Bulk

This Mouse Adiponectin ELISA is used to measure & quantify Adiponectin levels in Metabolism & Endocrine research.

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EZMADP-60BK
10 Mouse Adiponection ELISA Kits (EZMADP-60K)  
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      개요

      Replacement Information
      Replacement InformationThis product replaces CYT286, CHEMICON® Mouse Adiponectin ELISA Kit
      Description
      Catalogue NumberEZMADP-60BK
      ReplacesCYT286
      DescriptionMouse Adiponectin ELISA- Bulk
      Background InformationAdiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
      References
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: 1.4–10.8
      • Intra-assay: 3.8–8.2
      Quality LevelMQ200
      Applications
      ApplicationThis Mouse Adiponectin ELISA is used to measure & quantify Adiponectin levels in Metabolism & Endocrine research.
      Key Applications
      • ELISA
      Application NotesRoom temperature, 3 hour assay 20 μL sample size (serum/plasma: 1:1000 dilution)
      Biological Information
      Species Reactivity
      • Mouse
      Analytes Available
      • Adiponectin
      Entrez Gene SummaryThis gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
      Gene Symbol
      • ADIPOQ
      • ACDC
      • ACRP30
      • APM1
      • GBP28
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
      SIZE: 244 amino acids; 26414 Da
      SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
      PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
      DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.
      SIMILARITY: Contains 1 C1q domain. & Contains 1 collagen-like domain.
      MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. & HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW- complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls.
      Accuracy
      • 99–112%
      Physicochemical Information
      Sensitivity
      • 0.78 ng/mL
      Linearity of Dilution96–121%
      Standard Curve Range
      • 0.78–50 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material Size10 Mouse Adiponection ELISA Kits (EZMADP-60K)
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      EZMADP-60BK 04053252437021

      Documentation

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