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Merck

FCMAB116P

Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70

clone MC-813-70, Milli-Mark®, from mouse

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제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
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제품 이름

Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70, clone MC-813-70, Milli-Mark®, from mouse

biological source

mouse

conjugate

PE

antibody form

purified antibody

antibody product type

primary antibodies

clone

MC-813-70, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable

isotype

IgG3κ

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... FUT4(2526)

관련 카테고리

Analysis Note

Control
Pluripotent human embryonic stem (ES) cells

Application

Immunocytochemical staining of live H9 human ES cells incubated for 30 minutes at 37oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugate (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Immunocytochemical staining of fixed H9 human ES cells incubated for 2 hours at 2-8oC with 1:50 dilution of anti-SSEA-4, clone MC-813-70, PE conjugated (Cat. No. FCMAB116P) monoclonal antibody.
Pluripotent human ES cells exhibit strong immunoreactivity to this antibody.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
This Milli-Mark Anti-SSEA-4-PE Antibody, clone MC-813-70 is validated for use in FC, IC for the detection of SSEA-4.

Biochem/physiol Actions

This antibody reacts with the stage-specific embryonic antigen-4 (SSEA-4) that is expressed upon the surface of human tetracarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES).
No immunoreactivity is evident with undifferentiated murine EC, ES and EG cells. Expression of SSEA-4 is down regulated following differentiation of human EC cells. In ctontrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-terminal GST-tagged, recombinant human MuRF1 full length, expressed in E.coli. Purified using glutathione sepharose.
The cell-surface markers used routinely to identify undifferentiated human embryonic stem cells (hESCs) were initially characterized as markers for mESCs, mouse embryonic carcinomas (ECs), or human EC cells. Stage-specific embryonic antigen-3 (SSEA-3) and SSEA-4 are widely used as cell-surface markers to define both human and mouse ESCs. SSEA-3 and SSEA-4 are expressed on undifferentiated hESCs but not on undifferentiated mESCs.

Immunogen

Human embryonal carcinoma cell line 2102Ep.

Physical form

Protein A purified
Purified mouse monoclonal IgG3 conjugated to PE fluorochrome in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

Preparation Note

Maintain refrigerated at 2-8oC protected from light in undiluted aliquots for up to 6 months from date of receipt.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

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저장 등급

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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문서 라이브러리 방문

Pooja Biswas et al.
Human mutation, 42(2), 189-199 (2020-12-01)
Inherited retinal degenerations (IRDs) are a group of genetically heterogeneous conditions with a broad phenotypic heterogeneity. Here, we report detection and validation of the underlying cause of progressive retinal degeneration in a nuclear family of European descent with a single
Vanessa Sauer et al.
Cell transplantation, 25(12), 2221-2243 (2016-08-12)
Although several types of somatic cells have been reprogrammed into induced pluripotent stem cells (iPSCs) and then differentiated to hepatocyte-like cells (iHeps), the method for generating such cells from renal tubular epithelial cells shed in human urine and transplanting them

국제 무역 품목 번호

SKUGTIN
FCMAB116P04053252371301

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