제품 이름
LiChrospher® 100 RP-8 (5 µm) LiChroCART™ 125-4, suitable for HPLC
material
stainless steel column
Quality Segment
agency
suitable for USP L7
product line
LiChrospher®
feature
endcapped: no
extent of labeling
12.5% Carbon loading
parameter
250 bar pressure, 60 °C max. temp.
technique(s)
HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
12.5 cm × 4 mm
surface area
350 m2/g
matrix
fully porous particle
matrix active group
C8 (RP8), C8 bonding phase
particle size
5 μm
pore size
100 Å pore size
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
General description
HPLC cartridge
LiChrospher® RP-8 is a reliable and versatile traditionally produced spherical silica gel carrier with C-8 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 125-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).
Application
- Determination of dopamine, noradrenaline, and adrenaline in Krebs-Henseleit solution by liquid chromatography coupled with tandem mass spectrometry: This study showcases the use of LiChrospher® 100 RP-8 (5 µm) in a LICHROCART™ 125-4 setup for precise neurotransmitter measurement in biological samples, advancing understanding in neurochemistry and cardiovascular research. The column′s selectivity and resolution enhance the detection of these small molecules, pivotal for biochemical assays and physiological studies (Britto-Júnior et al., 2021).
Analysis Note
Pressure: ≤ 45 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.8 - 2.0
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.8 - 2.0
Legal Information
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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저장 등급
11 - Combustible Solids
시험 성적서(COA)
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