Millipore Sigma Vibrant Logo

70796 His•Tag® Monoclonal Antibody

Sensitive, specific detection of His•Tag fusion proteins

His•Tag® Monoclonal Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
70796
가격 및 재고 조회

개요

Replacement Information

가격 및 재고여부

카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
70796-3CN
재고 정보 검색...
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      		 Plastic ampoule 100 μg
      가격 검색...
      가격을 검색할 수 없습니다
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      가격 문의 추가 정보
      ()이 할인됨
       
      —
      가격 문의
      		 —
      70796-4CN
      재고 정보 검색...
      —
      현재 재고 없음 현재 재고 없음
      예상 출고 가능일 
      단종품
      제한된 수량 가능
      재고여부 확인
        Remaining: will advise
          Remaining: will advise
          추천사항
          고객 서비스로 문의
          Contact Customer Service

          		 Plastic ampoule 3 μg
          가격 검색...
          가격을 검색할 수 없습니다
          Minimum Quantity needs to be mulitiple of
          Maximum Quantity is
          가격 문의 추가 정보
          ()이 할인됨
           
          —
          가격 문의
          		 —
          Description
          Overview

          The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of the Novagen® expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10-8 to 1 × 10-9 M) enables sensitive, specific detection of His•Tag fusion proteins at antibody concentrations of 0.1-0.2 µg/ml. The His•Tag Monoclonal Antibody binds to N-terminal, C-terminal, and internal His•Tag sequences. This antibody also detects the recombinant marker bands in the Perfect Protein™ and Trail Mix™ Western Markers for convenient visualization of accurate internal standards on Western blots*. For lowest background in Western blotting applications, Alkali-soluble Casein is recommended as a blocking agent. The 100-µg package size provides enough purified antibody for 1000 ml of working solution.

          * Not recommended for HRP-based detection of Trail Mix Western Markers.
          Catalogue Number70796
          Brand Family Novagen®
          References
          Product Information
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          카탈로그 번호 GTIN
          70796-3CN 04055977272512
          70796-4CN 04055977272529

          Documentation

          His•Tag® Monoclonal Antibody MSDS

          타이틀

          물질안전보건자료(MSDS) 

          His•Tag® Monoclonal Antibody Certificates of Analysis

          TitleLot Number
          70796

          Citations

          타이틀
        • Alexander Schwendemann, et al. (2008) Hip, an HP1-interacting protein, is a haplo- and triplo-suppressor of position effect variegation. Procedings of the National Academy of Science 105, 204-209.
        • Antonella Antignani and Richard J. Youle. (2007) The cytokine, granulocyte-macrophage colony-stimulating factor (GM-CSF), can deliver Bcl-XL as an extracellular fusion protein to protect cells from apoptosis and retain differentiation induction. Journal of Biological Chemistry 282, 11246-11254.
        • Valentina A. Shchedrina, et al. (2007) Identification and characterization of a selenoprotein family containing a diselenide bond in a redox motif. Procedings of the National Academy of Science 104, 13919-13924.
        • Claire Vincent, et al. (2007) Activation of p61Hck triggers WASp- and Arp2/3-dependent actin-comet tail biogenesis and accelerates lysosomes. Journal of Biological Chemistry 282, 19565-19574.
        • Wilfried Weber, et al. (2007) A synthetic time-delay circuit in mammalian cells and mice. Procedings of the National Academy of Science 104, 2643-2648.
        • Martin Gengenbacher, et al. (2006) Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights. Journal of Biological Chemistry 281, 3633-3641.
        • Michael Krauss, et al. (2006) Stimulation of phosphatidylinositol kinase type I-mediated phosphatidylinositol (4,5)-bisphosphate synthesis by AP-2µ-cargo complexes. Procedings of the National Academy of Science 103, 11934-11939.
        • Luis Ojeda, et al. (2006) Role of glutaredoxin-3 and glutaredoxin-4 in the iron regulation of the Aft1 transcriptional activator in Saccharomyces cerevisiae. Journal of Biological Chemistry 281, 17661-17669.
        • Brian Adams, et al. (2005) A novel class of dual-family immunophilins. Journal of Biological Chemistry 280, 24308-24314.
        • Karl J. Aichberger, et al. (2005) Hom s 4, an IgE-reactive autoantigen belonging to a new subfamily of calcium-binding proteins, can induce Th cell type 1-mediated autoreactivity. Journal of Immunology 175, 1286-1294.
        • Jessica L. Anderson, et al. (2005) Purification, functional reconstitution and characterization of the Saccharomyces cerevisiae isoprenylcysteine carboxyl methyltransferase Ste14p. Journal of Biological Chemistry 280, 7336-7345.
        • Erika Crouch, et al. (2005) Ligand specificity of human surfactant protein D: expression of a mutant trimeris collection that shows enhanced interactions with influenza A virus. Journal of Biological Chemistry 280, 17046-17056.
        • Chungho Kim, et al. (2005) Filamin is essential for shedding of the transmembrane serine protease, epithin. European Molecular Biology Organization Reports 6, 1045-1051.
        • Dehe Kong, et al. (2005) Echinomycin, a small-molecule inhibitor of hypoxia-inducible factor-1 DNA-binding activity. Cancer Research 65, 9047-9055.
        • Alla Korepanova, et al. (2005) Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli. Protein Science 14, 148-158.
        • Zuzanna Kwade, et al. (2005) Identification of four adenosine kinase isoforms in tobacco by-2 cells and their putative role in the cell cycle regulated cytokinin metabolism. Journal of Biological Chemistry 280, 17512-17519.
        • Nikolay L. Malinin, et al. (2005) Amyloid-b neurotoxicity is mediated by FISH adapter protein and ADAM12 metalloprotease activity. Proceedings of the National Academy of Sciences (USA) 102, 3058-3063.
        • Kyoung Joon Oh, et al. (2005) Conformational changes in BID, a pro-apoptotic BCL-2 family member, upon membrane binding: a site-directed spin labeling study. Journal of Biological Chemistry 280, 753-767.
        • Kazuki Okamoto and Fumihide Isohashi. (2005) Macromolecular-translocation inhibitor-II (Zn2+-binding protein, parathymosin) interacts with the glucocorticoid receptor and enhances transcription in vivo. Journal of Biological Chemistry 280, 36986-36993.
        • Michael R Taylor, et al. (2005) The zebrafish pob gene encodes a novel protein required for survival of red cone photoreceptor cells. Genetics 170, 263-273.
        • Ronnie R. Wei, Peter K. Sorger and Stephen C. Harrison. (2005) Molecular organization of the Ndc80 complex, an essential kinetochore component. Proceedings of the National Academy of Sciences (USA) 102, 5363-5367.
        • Tania A. Wong, et al. (2005) Membrane metabolism mediated by Sec14 family members influences Arf GTPase activating protein activity for transport from the trans-Golgi. Proceedings of the National Academy of Sciences (USA) 102, 12777-12782.
        • Aileen Aherne, et al. (2004) On the molecular pathology of neurodegeneration in IMPDH1-based retinitis pigmentosa. Human Molecular Genetics 13, 641-650.
        • F. Bühling, et al. (2004) Simultaneous detection and differentiation of anti-Helicobacter pylori antibodies by flow microparticle immunofluorescence assay. 11, 131-136.
        • Carlos Gorbea, et al. (2004) Characterization of mammalian Ecm29, a 26 S proteasome-associated protein that localizes to the nucleus and membrane vesicles. Journal of Biological Chemistry 279, 54849-54861.
        • Lei Jin, et al. (2004) The three-dimensional structure of the ZAP-70 kinase domain in complex with staurosporine implications for the design of selective innhibitors. Journal of Biological Chemistry 279, 42818-42825.
        • Yuanhe Li, et al. (2004) Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes. European Molecular Biology Organization Journal 23, 2723-2733.
        • Katerina Ragkousi, et al. (2003) Identification of a new gene essential for germination of Bacillus subtilis spores with Ca2+-dipicolinate. Journal of Bacteriology 185, 2315-2329.
        • Evelyne C. Deery, et al. (2002) Disease mechanism for retinitis pigmentosa (RP11) caused by mutations in the splicing factor gene PRPF31. Human Molecular Genetics 11, 3209-3219.
        • Nan Zhang, et al. (1997) Human breast cancer growth inhibited in vivo by a dominant negative pleiotrophin mutant. Journal of Biological Chemistry 272, 16733-16736.
          		•

          User Protocols

          Title
          TB283 His•Tag® Monoclonal Antibody