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354103 Glutathione Assay Kit II

354103
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개요

주요 사양표

Detection Methods
Colorimetric

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354103-1KITCN
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      Glass bottle 1 kit
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      Description
      OverviewA sensitive and convenient colorimetric (414 or 405 nm) assay kit for the measurement of glutathione from plasma, serum, erythrocyte lysate, tissue samples, and cultured cells. Dynamic range: 0 - 16 µM for GSH or 0 - 8 µM for GSSG. For plasma and serum samples concentration and deproteination are required.
      Catalogue Number354103
      Brand Family Calbiochem®
      Application Data


      Materials Required but Not Delivered A plate reader with a 414 or 405 nm filter.
      An adjustable pipettor, repeat pipettor, and an eight-channel pipettor (optional).
      A source of pure water. Glass distilled water or HPLC-grade water is acceptable.
      Metaphosphoric acid and triethanolamine (described in Recommended procedure for deproteination of samples).
      2-vinylpyridine (optional, described in Sample preparation for exclusive measurement of GSSG).
      References
      ReferencesFoyer, C.H., et al. 1994. Physiol. Plant. 92, 696.
      Baillie, T.A. and Slatter, J.G. 1991. Acc. Chem. Res. 24, 264.
      Baker, M.A., et al. 1990. Anal. Biochem. 190, 360.
      Inoue, M., et al. 1987. Journal of Protein Chemistry 6, 207.
      Eyer, P. and Podhradsky, D. 1986. Anal. Biochem. 153, 57.
      Inoue, M. 1985. in Renal Biochemistry. Kinne, R.K.H. editor. Elsevier Science Publishers B.V. London, 225.
      Lash, L.H. and Jones, D.P. 1985. Arch. Biochem. Biophys. 240, 583.
      Griffith, O.W. 1980. Anal. Biochem. 106, 207.
      Wendel, A. and Cikryt, P. 1980. FEBS Lett. 120, 209.
      Glutathione: Metabolism and function, Arias, I.M. and Jakoby, W.B. 1976. editors. Raven Press, New York.
      Tietze, F. 1969. Anal. Biochem. 27, 502.
      Product Information
      Detection methodColorimetric
      Form96 Tests
      Format96-well plate
      Kit contains2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.
      Positive controlGSSG
      Quality LevelMQ100
      Biological Information
      Assay range 0 - 16 µM for GSH or 0 - 8 µM for GSSG
      Assay time3 h
      Sample TypePlasma, serum, erythrocyte lysate, tissue samples, and cultured cells
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store entire kit contents at 4°C.
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit contains2X MES Buffer, GSSG Standard, Cofactor Mixture, Enzyme Mixture, DTNB, 96-Well Plate, Plate Cover, and a user protocol. Assay requires Metaphosphoric Acid and Triethanolamine to be supplied by the user.
      Global Trade Item Number
      카탈로그 번호 GTIN
      354103-1KITCN 04055977193282