FlowCellect Oxidative Stress Characterization Kit

Research Category
Neuroscience

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Oxidative stress has been found to play a key role in a number of pathological disorders. To aid researchers studying this phenomenon Millipore has developed the FlowCellect Oxidative Stress Characterization Kit. This kit contains the chemical and immunological reagents necessary to detect carbonyl groups introduced onto proteins by reactive oxygen species (ROS). The test method involves derivatization of protein carbonyl groups with 2,4-dinitrophenylhydrazine (DNPH). This chemical reaction results in proteins being covalently coupled to DNP at their carbonyl sites. The DNP-derivatized proteins are then incubated with a FITC conjugated monoclonal antibody that specifically binds to the DNP moiety. Subsequent detection with a bench-top Guava EasyCyte Plus flow cytometer allows quantification of the extent of oxidative stress within cells. Although the assay and all of the kit components were optimized using a Guava instrument, any flow cytometer can be utilized provided it contains a blue laser (488 nm) light source.

Anti-DNP -FITC

Derivatization Control Solution

5X Assay Buffer

DNPH Solution

10X Wash Buffer

User Manual

Upon arrival the FITC conjugated anti-DNP antibody should be transferred to a -20°C freezer. The remaining kit components (FCCH025111-1) should be stored at 2 - 8ºC.
All kit components are stable for up to six (6) months from date of receipt if stored and handled appropriately.

FlowCellect is a trademark of Luminex Corporation