다음 MAP메이트™는 통합될 수 없습니다: -다른 분석 완충용액이 필요한 MAP메이트™. -인산 특이성 및 총 MAP메이트™ 조합, 예: 총 GSK3β 및 GSK3β(Ser 9). -PanTyr 및 자리 특이성 MAP메이트™, 예: Phospho-EGF 수용체 및 phospho-STAT1(Tyr701). -단일 표적(Akt, STAT3)를 위한 1개 이상의 1 phospho-MAP메이트™. - GAPDH 및 β-Tubulin은 panTyr를 포함하는 키트 또는 MAP메이트™와 통합될 수 없습니다.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
이 제품은 즐겨찾기에 저장되었습니다.
종
패널 유형
선택하신 키트
수량
카탈로그 번호
주문 설명
포장 단위
기재 가격
96-Well Plate
수량
카탈로그 번호
주문 설명
포장 단위
기재 가격
다른 시약 추가 (MAP메이트 사용을 위해 완충용액과 검출 키트가 필요함)
수량
카탈로그 번호
주문 설명
포장 단위
기재 가격
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
공간 절약 옵션 다수의 키트를 구매하시는 고객은 고용량 저장을 위해 키트 포장을 제거하고 비닐백에 담긴 멀티플레스 분석 구성품을 받아 저장 공간을 절약하도록 선택할 수 있습니다.
이 제품은 즐겨찾기에 저장되었습니다.
해당 제품은 고객님의 카트에 추가되었습니다.
이제 다른 키트를 사용자 지정하거나, 사전 혼합된 키트를 선택하거나, 결재하거나 또는 주문 도구를 종료할 수 있습니다.
Please refer to vial label for lot-specific concentration
Physicochemical Information
Contaminants
None detected by IEP using anti-fibronectin and anti-rat serum
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Dry Ice Only
Toxicity
Standard Handling
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
02-May-2008 RFH
Description
Native fibronectin purified from pooled rat plasma. Effective agent for promoting attachment of cells to commonly-used culture substrates.
Form
Liquid
Formulation
In 100 mM NaCl, 20 mM sodium phosphate buffer, pH 7.3, preservative and reductant-free.
Concentration Label
Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Procedure for Coating of Tissue Culture Plates with Fibronectin
This procedure is based on the use of 21 cm2 dishes (Corning 25010, Costar 3060, Falcon 3002, or an equivalent 60 mm dish) and the 1 mg package size of fibronectin. One mg of fibronectin is sufficient to coat 10 dishes at 5 µg/cm2.
1. After thawing the fibronectin solution as indicated above, bring the solution to a final volume of 20 ml with sterile serum-free medium that has been preheated to 37°C. This yields a fibronectin solution of 50 µg/ml.
2. Add 2 ml of this solution to each of 10 tissue culture plates and swirl gently to completely coat the entire growth surface.
3. Allow dishes to remain at room temperature for ~45 min to permit binding of the fibronectin to the growth surfaces.
4. Tilt each dish and remove the fibronectin solution using a sterile pipette. Do not permit the pipette tip to disturb the growth surface.
5. Add cell suspension in medium directly to the fibronectin-coated dishes.
This procedure is meant to serve as a guideline and optimal may require optimization.
CAS number
86088-83-7
Purity
single major band by SDS-PAGE
Contaminants
None detected by IEP using anti-fibronectin and anti-rat serum
Storage
Avoid freeze/thaw
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C). Be VERY careful during the thawing process. Place the vial in a 37°C water bath and leave it there until it is completely thawed. DO NOT DISTURB OR REMOVE THE VIAL AT ANY TIME DURING THE THAWING PERIOD. If the vial is disturbed or removed prior to complete thawing, the product will gel and be unusable. DO NOT VORTEX. DO NOT SHAKE AFTER THAWING. Mix very gently after thawing.